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18 April 2017 Assessment of decellularization of heart bioimplants using a Raman spectroscopy method
Elena V. Timchenko, Pavel E. Timchenko, Artur Lichtenberg, Alexander Assmann, Hug Aubin, Payam Akhyari, Larisa T. Volova, Svetlana V. Pershutkina
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Journal of Biomedical Optics, Vol. 22, Issue 9, 091511 (April 2017). https://doi.org/10.1117/1.JBO.22.9.091511
Abstract
We report the results of experimental studies on cardiac implants using a Raman spectroscopy method (RS). Raman spectra characteristics of leaves and walls of cardiac implants were obtained; the implants were manufactured by protocols of detergent-enzymatic technique (DET) and biological, detergent-free (BIO) decellularization, using detergents (group DET) or a detergent-free, nonproteolytic, actin-disassembling regimen (BIO). There were input optical coefficients that allowed us to carry out evaluation of the protocols of DET and BIO decellularization on the basis of the concentrations of glycosaminoglycans, proteins, amides, and DNA. It was shown that during DET and BIO decellularization, composition aberrations of proteins and lipids do not occur and the integrity of the collagenous structures is preserved. It was found that during the DET decellularization, preservation of glycosaminoglycans is better than during BIO decellularization.
© 2017 Society of Photo-Optical Instrumentation Engineers (SPIE) 1083-3668/2017/$25.00 © 2017 SPIE
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Elena V. Timchenko, Pavel E. Timchenko, Artur Lichtenberg, Alexander Assmann, Hug Aubin, Payam Akhyari, Larisa T. Volova, and Svetlana V. Pershutkina "Assessment of decellularization of heart bioimplants using a Raman spectroscopy method," Journal of Biomedical Optics 22(9), 091511 (18 April 2017). https://doi.org/10.1117/1.JBO.22.9.091511
Received: 3 December 2016; Accepted: 22 March 2017; Published: 18 April 2017
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Cited by 16 scholarly publications.
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KEYWORDS
Raman spectroscopy
Heart
Proteins
Tissues
Matrices
Raman scattering
Remote sensing
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