Automated classification of multiphoton microscopy images of ovarian tissue using deep learning

Mikko J. Huttunen; Abdurahman Hassan; Curtis W. McCloskey; Sijyl Fasih; Jeremy Upham; Barbara C. Vanderhyden; Robert W. Boyd; Sangeeta Murugkar

doi:10.1117/1.JBO.23.6.066002

13 June 2018 Automated classification of multiphoton microscopy images of ovarian tissue using deep learning

Mikko J. Huttunen, Abdurahman Hassan, Curtis W. McCloskey, Sijyl Fasih, Jeremy Upham, Barbara C. Vanderhyden, Robert W. Boyd, Sangeeta Murugkar

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Journal of Biomedical Optics, Vol. 23, Issue 6, 066002 (June 2018). https://doi.org/10.1117/1.JBO.23.6.066002

Abstract

Histopathological image analysis of stained tissue slides is routinely used in tumor detection and classification. However, diagnosis requires a highly trained pathologist and can thus be time-consuming, labor-intensive, and potentially risk bias. Here, we demonstrate a potential complementary approach for diagnosis. We show that multiphoton microscopy images from unstained, reproductive tissues can be robustly classified using deep learning techniques. We fine-train four pretrained convolutional neural networks using over 200 murine tissue images based on combined second-harmonic generation and two-photon excitation fluorescence contrast, to classify the tissues either as healthy or associated with high-grade serous carcinoma with over 95% sensitivity and 97% specificity. Our approach shows promise for applications involving automated disease diagnosis. It could also be readily applied to other tissues, diseases, and related classification problems.

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Mikko J. Huttunen, Abdurahman Hassan, Curtis W. McCloskey, Sijyl Fasih, Jeremy Upham, Barbara C. Vanderhyden, Robert W. Boyd, and Sangeeta Murugkar "Automated classification of multiphoton microscopy images of ovarian tissue using deep learning," Journal of Biomedical Optics 23(6), 066002 (13 June 2018). https://doi.org/10.1117/1.JBO.23.6.066002

Received: 22 March 2018; Accepted: 31 May 2018; Published: 13 June 2018

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