Microscopy with ultraviolet surface excitation for wide-area pathology of breast surgical margins

Weisi Xie; Ye Chen; Yu Wang; Linpeng Wei; Chengbo Yin; Adam K. Glaser; Mark E. Fauver; Eric J. Seibel; Suzanne M. Dintzis; Joshua C. Vaughan; Nicholas P. Reder; Jonathan T. C. Liu

doi:10.1117/1.JBO.24.2.026501

8 February 2019 Microscopy with ultraviolet surface excitation for wide-area pathology of breast surgical margins

Weisi Xie, Ye Chen, Yu Wang, Linpeng Wei, Chengbo Yin, Adam K. Glaser, Mark E. Fauver, Eric J. Seibel, Suzanne M. Dintzis, Joshua C. Vaughan, Nicholas P. Reder, Jonathan T. C. Liu

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Journal of Biomedical Optics, Vol. 24, Issue 2, 026501 (February 2019). https://doi.org/10.1117/1.JBO.24.2.026501

Abstract

Intraoperative assessment of breast surgical margins will be of value for reducing the rate of re-excision surgeries for lumpectomy patients. While frozen-section histology is used for intraoperative guidance of certain cancers, it provides limited sampling of the margin surface (typically <1 % of the margin) and is inferior to gold-standard histology, especially for fatty tissues that do not freeze well, such as breast specimens. Microscopy with ultraviolet surface excitation (MUSE) is a nondestructive superficial optical-sectioning technique that has the potential to enable rapid, high-resolution examination of excised margin surfaces. Here, a MUSE system is developed with fully automated sample translation to image fresh tissue surfaces over large areas and at multiple levels of defocus, at a rate of ∼5 min / cm². Surface extraction is used to improve the comprehensiveness of surface imaging, and 3-D deconvolution is used to improve resolution and contrast. In addition, an improved fluorescent analog of conventional H&E staining is developed to label fresh tissues within ∼5 min for MUSE imaging. We compare the image quality of our MUSE system with both frozen-section and conventional H&E histology, demonstrating the feasibility to provide microscopic visualization of breast margin surfaces at speeds that are relevant for intraoperative use.

CC BY: © The Authors. Published by SPIE under a Creative Commons Attribution 4.0 Unported License. Distribution or reproduction of this work in whole or in part requires full attribution of the original publication, including its DOI.

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Weisi Xie, Ye Chen, Yu Wang, Linpeng Wei, Chengbo Yin, Adam K. Glaser, Mark E. Fauver, Eric J. Seibel, Suzanne M. Dintzis, Joshua C. Vaughan, Nicholas P. Reder, and Jonathan T. C. Liu "Microscopy with ultraviolet surface excitation for wide-area pathology of breast surgical margins," Journal of Biomedical Optics 24(2), 026501 (8 February 2019). https://doi.org/10.1117/1.JBO.24.2.026501

Received: 1 December 2018; Accepted: 21 January 2019; Published: 8 February 2019

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