Portable low-cost macroscopic mapping system for all-optical cardiac electrophysiology

Yuli W. Heinson; Julie L. Han; Emilia Entcheva

doi:10.1117/1.JBO.28.1.016001

10 January 2023 Portable low-cost macroscopic mapping system for all-optical cardiac electrophysiology

Yuli W. Heinson, Julie L. Han, Emilia Entcheva

Author Affiliations +

Journal of Biomedical Optics, Vol. 28, Issue 1, 016001 (January 2023). https://doi.org/10.1117/1.JBO.28.1.016001

Abstract

Significance

All-optical cardiac electrophysiology enables the visualization and control of key parameters relevant to the detection of cardiac arrhythmias. Mapping such responses in human induced pluripotent stem-cell-derived cardiomyocytes (hiPSC-CMs) is of great interest for cardiotoxicity and personalized medicine applications.

Aim

We introduce and validate a very low-cost compact mapping system for macroscopic all-optical electrophysiology in layers of hiPSC-CMs.

Approach

The system uses oblique transillumination, low-cost cameras, light-emitting diodes, and off-the-shelf components (total < $15 , 000) to capture voltage, calcium, and mechanical waves under electrical or optical stimulation.

Results

Our results corroborate the equivalency of electrical and optogenetic stimulation of hiPSC-CMs, and V_m − [ Ca^{2 +}_]i similarity in conduction under pacing. Green-excitable optical sensors are combinable with blue optogenetic actuators (chanelrhodopsin2) only under very low green light (<0.05 mW/mm²). Measurements in warmer culture medium yield larger spread of action potential duration and higher conduction velocities compared to Tyrode’s solution at room temperature.

Conclusions

As multiple optical sensors and actuators are combined, our results can help handle the “spectral congestion” and avoid parameter distortion. We illustrate the utility of the system for uncovering the action of cellular uncoupling agents and show extensibility to an epi-illumination mode for future imaging of thicker native or engineered tissues.

CC BY: © The Authors. Published by SPIE under a Creative Commons Attribution 4.0 International License. Distribution or reproduction of this work in whole or in part requires full attribution of the original publication, including its DOI.

Citation Download Citation

Yuli W. Heinson, Julie L. Han, and Emilia Entcheva "Portable low-cost macroscopic mapping system for all-optical cardiac electrophysiology," Journal of Biomedical Optics 28(1), 016001 (10 January 2023). https://doi.org/10.1117/1.JBO.28.1.016001

Received: 14 September 2022; Accepted: 19 December 2022; Published: 10 January 2023

ACCESS THE FULL ARTICLE

JOURNAL ARTICLE
16 PAGES

DOWNLOAD PAPER SAVE TO MY LIBRARY