Quick tour of fluorescence correlation spectroscopy from its inception

Elliot L. Elson

doi:10.1117/1.1779234

1 September 2004 Quick tour of fluorescence correlation spectroscopy from its inception

Elliot L. Elson

Author Affiliations +

Journal of Biomedical Optics, Vol. 9, Issue 5, (September 2004). https://doi.org/10.1117/1.1779234

Abstract

Fluorescence correlation spectroscopy (FCS) was originally developed in the early 1970s as a way to measure the kinetics of chemical reactions under zero perturbation conditions. At its inception, the measurement was difficult due to experimental limitations and was primarily used during the 1970s and 1980s to characterize diffusion. More recently, as a result of technological advances, FCS measurements have become easier and more versatile. In addition to measurements of diffusion both in solution and in cells, FCS is now also used to measure not only chemical reaction kinetics but also extents of molecular aggregation, the dynamics of photophysical processes, conformational fluctuations, molecular interactions in solution and in cells, and has even found application as a pharmaceutical screening method. From its inception to the present, the contributions of Webb and his coworkers have had a central and defining role in the development and applications of FCS.

©(2004) Society of Photo-Optical Instrumentation Engineers (SPIE)

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Elliot L. Elson "Quick tour of fluorescence correlation spectroscopy from its inception," Journal of Biomedical Optics 9(5), (1 September 2004). https://doi.org/10.1117/1.1779234

Published: 1 September 2004

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KEYWORDS

Fluorescence correlation spectroscopy

Physics

Biomedical optics

Multiphoton microscopy

Absorption

Chemistry

Luminescence

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