19 July 2022 Imaging of eosin-stained brain section using two-photon excitation fluorescence microscopy
Guozhong Hou, Zhiwei Dong, Sheng Zhang, Qiqi Bai, Shuo Liu, Yuanqin Xia
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Abstract

A two-photon excitation fluorescence (TPEF) microscopy system under broadband excitation of femtosecond pulses is built in this study. The TPEF spectrum of an eosin stain is recorded, and the TPEF microscopy system is equipped with a suitable detection window matching the TPEF spectrum of the eosin stain. TPEF microscopy is demonstrated using a solid eosin sample and eosin-stained mouse brain sections through detecting the TPEF signal from eosin staining. Three-dimensional (3D) reconstruction of the mouse brain section across its thickness is realized with several TPEF imaging frames acquired via z-axis scanning with a step size of 1 μm. Two-dimensional TPEF imaging frames and 3D reconstruction of the brain section demonstrated high performance of TPEF microscopy in the observation of eosin-stained biological sections.

© 2022 Society of Photo-Optical Instrumentation Engineers (SPIE)
Guozhong Hou, Zhiwei Dong, Sheng Zhang, Qiqi Bai, Shuo Liu, and Yuanqin Xia "Imaging of eosin-stained brain section using two-photon excitation fluorescence microscopy," Optical Engineering 61(7), 073103 (19 July 2022). https://doi.org/10.1117/1.OE.61.7.073103
Received: 1 April 2022; Accepted: 28 June 2022; Published: 19 July 2022
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Cited by 1 scholarly publication.
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KEYWORDS
Brain

Microscopy

Neuroimaging

Solids

Luminescence

Imaging systems

Spatial resolution

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