An imaging device based on line-field confocal optical coherence tomography (LC-OCT) operating in two distinct spectral bands centered at 770 nm and 1250 nm is presented. A single supercontinuum light source and two different line-scan cameras are used. B-scans are acquired simultaneously in the two bands at 4 frames per second. In the 770-nm band, high resolution (1.3 μm x 1.2 μm, lateral x axial) imaging is achieved, while extended penetration (~ 700 μm) is obtained in the 1250-nm band. Greyscale fusion of the two images is performed to produce a single image with both high resolution in the superficial part of the image and deep penetration. A color representation is also used to highlight spectroscopic properties of the sample and to enhance contrast.
We present an improved time-domain optical coherence tomography technique designed for ultrahigh-resolution B-scan imaging in real-time. The technique, called line-field confocal optical coherence tomography, is based on a Linnik-type interference microscope with line illumination using a supercontinuum laser and line detection using a line-scan camera. Bscan imaging with dynamic focusing is achieved by acquiring multiple A-scans in parallel. In vivo cellular level resolution imaging of skin is demonstrated at 10 frame/s with a penetration depth of ∼ 500 μm, with a spatial resolution of 1.3 μm × 1.1 μm (transverse × axial).
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