KEYWORDS: Skin, In vivo imaging, Associative arrays, Tumors, Melanoma, Image resolution, Diagnostics, Denoising, Convolutional neural networks, Chemical elements
Multiphoton microscopy can provide sub-micron resolution images of living tissues in their
native environment with chemical contrast. We recently reported on a fast large area multiphoton
exoscope (FLAME) for rapidly mapping out macroscopic tissue areas (cm-scale) with microscopic
resolution. In this presentation we demonstrate the imaging capability and the clinical utility of this
system by performing a pilot study on ex vivo imaging of benign and malignant pigmented lesions of
human skin. We identify morphological features such as cytological atypia, lentiginous hyperplasia,
migration of melanocytes and demonstrate the value of sampling large tissue volumes for capturing the
lesion heterogeneity.
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