Longitudinal imaging of live organisms is essential to life science research aiming to understand biological processes. Recently, the imaging system has been housed for longitudinal imaging by mounting the optical imaging system in an incubator, while facing the limitations of single-modality and low throughput. Here, we proposed the OCToScope, a multi-modal, high-throughput and compact platform that is compatible with commercial incubators. OCToScope incorporates Optical Coherence Tomography(OCT) to achieve non-invasive three-dimensional imaging, and fluorescent imaging that retrieves cell-specific information. OCToScope also supports automatic scanning of mass samples quantity with motorized x-y-z stage and perform high time-resolution imaging for long-term studies. we used OCToScope to provide a systematic and comprehensive characterization of human heart organoids for over 30 days without any interference.
Organoids play an increasingly important role in in vitro models for studying organ development and disease mechanisms, and drug discovery. Recently, two groups independently developed human heart organoids from human pluripotent stem cells (hPSCs). In this study, we utilized a customized spectral-domain OCT (SD-OCT) to study heart organoids and demonstrated its capability to produce 3D images. Heart organoids formed cavities of various sizes, and complex interconnections were observed as early as on day 6. Heart organoids and the OCT system showed promising insights as an in vitro platform to investigate heart development and diseases mechanisms.
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