An innovative camera-based speckle contrast diffuse correlation tomography (scDCT) technology has been developed recently, which enables noncontact, noninvasive, high-density, 3D imaging of cerebral blood flow (CBF) distributions. This study demonstrated the capability and safety of scDCT technique for imaging of CBF distributions in a neonatal piglet model of transient ischemic stroke. Moreover, power spectral density analyses of low-frequency oscillations (LFOs) and the network connections over the brain were assessed before and after the induction of acute ischemic stroke. The stroke resulted in a substantial decrease in CBF, attenuations in resting-state LFOs, and functional connectivity disruptions in motor and somatosensory cortices.
KEYWORDS: Ischemia, Optical sensors, Simulation of CCA and DLA aggregates, Semiconductor lasers, Detector arrays, Spectroscopy, Speckle, Neuroscience, Laser tissue interaction, Head
We report an innovative, wearable, multiscale diffuse speckle contrast flowmetry (DSCF) probe for continuous transcranial imaging of cerebral blood flow (CBF) in animal s. Significant reductions in CBF during transient ligation of bilateral common carotid arteries were detected by DSCF (-35±13% in two mice and -59% in a piglet), meeting clinical expectations. Results from DSCF and an established CBF measurement device, diffuse correlation spectroscopy, were consistent and significantly correlated. With further optimization and validation in animals and humans, we expect to ultimately offer a unique, noninvasive, low-cost, and fast brain imaging tool for basic neuroscience research and clinical applications.
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