Although the mechanism of laser stimulation effect in bone has not completely understood, laser stimulation is
recommended in the treatment of osteoporosis due to positive treatment efficacy. In this study, a minimal invasive laser
needle system (MILNS) was developed using a fine hollow needle in order to stimulate directly bone site by guiding an
optical fiber. In order to evaluate the MILNS as a treatment method, in-vivo animal experiment study was performed
using osteopenic mice. Twelve virginal ICR mice were employed and divided two groups: SHAM-group and LASERgroup.
SHARM-group was stimulated by only fine hollow needle and LASER-group by fine hollow needle combined
with laser stimulation. All mice were served in-vivo micro-CT images before and after treatment. Three dimensional
(3D) structural parameters and vBMD (volume bone mineral density, g/cm3) in the trabecular bone were measured.
After 2 weeks of stimulation, the vBMD, BV/TV, Tb.Th and Tb.N in LASER-group were significantly higher than those
in SHAM-group (p<0.05). Potentially, this study suggested that the MILNS might prevent the bone loss and maintains
the bone mineral density of osteopenic mice.
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