Dr. Emmanuel Beaurepaire Profile

Dr. Emmanuel Beaurepaire

Research Director at Ecole Polytechnique

SPIE Involvement:

Author | Editor

Publications (33)

Proceedings Article | 1 September 2023 Paper

Paper

Color TSFG microscopy of red blood cells and oxygenation

Júlia Ferrer Ortas, Pierre Mahou, Sophie Escot, Chiara Stringari, Nicolas David, Laure Bally-Cuif, Nicolas Dray, Michel Négrerie, Willy Supatto, Emmanuel Beaurepaire

Proceedings Volume 12630, 1263002 (2023) https://doi.org/10.1117/12.2670229

KEYWORDS: Red blood cells, Oxygenation, Microscopy, Tissues, Absorption, Third harmonic generation, Sum frequency generation, Spatial resolution, Resonance enhancement, Multiphoton microscopy

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Proceedings Article | 25 April 2023 Presentation + Paper

Presentation + Paper

Simultaneous NAD(P)H and FAD fluorescence lifetime microscopy reveals long UVA–induced metabolic stress in reconstructed human skin

Thi Phuong Lien Ung, Seongbin Lim, Xavier Solinas, Pierre Mahou, Anatole Chessel, Claire Marionnet, Thomas Bornschlögl, Emmanuel Beaurepaire, Françoise Bernerd, Ana-Maria Pena, Chiara Stringari

Proceedings Volume 12384, 1238403 (2023) https://doi.org/10.1117/12.2646231

KEYWORDS: Skin, Fluorescence, Fluorescence lifetime imaging, Ultraviolet radiation, Microscopy, Mode conditioning cables, 3D modeling, Tunable filters, Tissues, Oxygen

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Proceedings Article | 20 May 2022 Presentation

Presentation

Nonlinear optical microscopy of developing and brain tissue

Emmanuel Beaurepaire

Proceedings Volume PC12136, PC1213601 (2022) https://doi.org/10.1117/12.2624475

KEYWORDS: Microscopy, Multiphoton microscopy, Brain, Tissues, Tissue optics, Optical microscopy, Image acquisition, Coherence imaging, Scattering, Neuroscience

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Proceedings Article | 7 December 2021 Paper

Paper

Fast cardiac imaging in live embryos using multiphoton light-sheet microscopy at low laser repetition rate

Vincent Maioli, Antoine Boniface, Pierre Mahou, Júlia Ferrer Ortas, Lamiae Abdeladim, Emmanuel Beaurepaire, Willy Supatto

Proceedings Volume 11922, 119220I (2021) https://doi.org/10.1117/12.2615701

KEYWORDS: Multiphoton microscopy, Microscopy, Heart, Cardiac imaging, Pulsed laser operation, Luminescence, Two photon imaging, Laser damage threshold, Beam splitters, Nonlinear optics

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Proceedings Article | 5 March 2021 Presentation

Presentation

Fast in vivo imaging using multiphoton light-sheet microscopy at optimal pulse frequency

Vincent Maioli, Antoine Boniface, Pierre Mahou, Júlia Ferrer Ortas, Lamiae Abdeladim, Emmanuel Beaurepaire, Willy Supatto

Proceedings Volume 11654, 116540E (2021) https://doi.org/10.1117/12.2578867

KEYWORDS: Multiphoton microscopy, Microscopy, Pulsed laser operation, In vivo imaging, Luminescence, Heart, Tissues, Structural dynamics, Spatial resolution

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Proceedings Article | 5 March 2021 Presentation + Paper

Presentation + Paper

Fast in vivo multiphoton microscopy using optimized light-sheet illumination

Vincent Maioli, Antoine Boniface, Pierre Mahou, Júlia Ferrer Ortas, Lamiae Abdeladim, Emmanuel Beaurepaire, Willy Supatto

Proceedings Volume 11648, 1164818 (2021) https://doi.org/10.1117/12.2578703

KEYWORDS: Multiphoton microscopy, In vivo imaging, Pulsed laser operation, Microscopy, Heart, Nonlinear optics, Femtosecond phenomena

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Proceedings Article | 22 July 2019 Paper

Paper

Chromatic serial multiphoton microscopy for multicolor imaging of large brain volumes

Lamiae Abdeladim, Katherine Matho, Solène Clavreul, Pierre Mahou, Jean-Marc Sintes, Xavier Solinas, Ignacio Arganda-Carreras, Anatole Chessel, Steve Turney, Jeff Lichtman, Alexis-Pierre Bemelmans, Karine Loulier, Willy Supatto, Jean Livet, Emmanuel Beaurepaire

Proceedings Volume 11076, 1107607 (2019) https://doi.org/10.1117/12.2526947

KEYWORDS: Multiphoton microscopy, Brain, Microscopy, Neuroimaging, Colorimetry, Tissues, Image resolution, Fluorescent proteins, Neurons, Image acquisition

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Proceedings Article | 22 July 2019 Paper

Paper

Label-free THG imaging of bone tissue microstructure: effect of low gravity on the lacuno-canalicular network

Rachel Genthial, Emmanuel Beaurepaire, Marie-Claire Schanne-Klein, Françoise Peyrin, Cécile Olivier, Maude Gerbaix, Laurence Vico, Aurélien Gourrier, Delphine Débarre

Proceedings Volume 11076, 110760E (2019) https://doi.org/10.1117/12.2527029

KEYWORDS: Bone, Second-harmonic generation, Tissues, Microscopy, Collagen, Biomedical optics, Tissue optics, Third-harmonic generation, Interfaces, 3D image processing

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Proceedings Article | 22 July 2019 Paper

Paper

Fast P-THG microscopy for the characterization of biomaterials

Joséphine Morizet, Guillaume Ducourthial, Willy Supatto, Arthur Boutillon, Renaud Legouis, Marie-Claire Schanne-Klein, Chiara Stringari, Emmanuel Beaurepaire

Proceedings Volume 11076, 1107613 (2019) https://doi.org/10.1117/12.2527209

KEYWORDS: Polarization, Microscopy, Anisotropy, Microscopes, In vivo imaging, Biological research, Multiphoton microscopy, Biomedical optics

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Proceedings Article | 14 March 2018 Presentation

Presentation

Metrology in nonlinear microscopy using harmonic generation nanoprobes (Conference Presentation)

Pierre Mahou, Guy Malkinson, Elodie Chaudan, Thierry Gacoin, Emmanuel Beaurepaire, Willy Supatto

Proceedings Volume 10498, 104981F (2018) https://doi.org/10.1117/12.2290328

KEYWORDS: Microscopes, Microscopy, Harmonic generation, Chromatic aberrations, Multiphoton microscopy, Metrology, Nanoprobes, Coherence imaging, Nanocrystals, Near infrared

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Proceedings Article | 5 March 2015 Paper

Paper

Multiphoton light-sheet microscopy using wavelength mixing: fast multicolor imaging of the beating Zebrafish heart with low photobleaching

Pierre Mahou, Julien Vermot, Emmanuel Beaurepaire, Willy Supatto

Proceedings Volume 9329, 93290Z (2015) https://doi.org/10.1117/12.2079176

KEYWORDS: Heart, Microscopy, Green fluorescent protein, Microscopes, Multiphoton microscopy, Cameras, Optical parametric oscillators, Mirrors, Chromophores, 3D acquisition

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Proceedings Article | 6 March 2013 Paper

Paper

Multiscale analysis of polarization-resolved third-harmonic generation microscopy from ordered lipid assemblies

Maxwell Zimmerley, Pierre Mahou, Delphine Débarre, Marie-Claire Schanne-Klein, Emmanuel Beaurepaire

Proceedings Volume 8622, 86220O (2013) https://doi.org/10.1117/12.2004175

KEYWORDS: Modulation, Skin, Microscopy, Polarization, Molecules, Nonlinear optics, Interfaces, Signal detection, Third-harmonic generation, Tissues

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Proceedings Article | 16 February 2012 Paper

Paper

Assessing correction accuracy in image-based adaptive optics

Delphine Débarre, Aurélie Facomprez, Emmanuel Beaurepaire

Proceedings Volume 8253, 82530F (2012) https://doi.org/10.1117/12.908808

KEYWORDS: Adaptive optics, Monochromatic aberrations, Sensors, Photons, Aberration correction, Microscopy, Wavefronts, Image quality, Confocal microscopy, Error analysis

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Proceedings Article | 3 February 2012 Paper

Paper

Correction accuracy in image-based adaptive optics for nonlinear microscopy

Aurélie Facomprez, Emmanuel Beaurepaire, Delphine Débarre

Proceedings Volume 8227, 822709 (2012) https://doi.org/10.1117/12.908647

KEYWORDS: Photons, Adaptive optics, Aberration correction, Microscopy, Sensors, Monochromatic aberrations, Evolutionary algorithms, Nonlinear optics, Luminescence, Computer simulations

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Proceedings Article | 3 February 2012 Paper

Paper

Calibration of an adaptive microscope using phase diversity

Delphine Débarre, Thibault Vieille, Aurélie Facomprez, Pierre Mahou, Emmanuel Beaurepaire

Proceedings Volume 8227, 822708 (2012) https://doi.org/10.1117/12.908632

KEYWORDS: Calibration, Objectives, Microscopes, Phase shift keying, Point spread functions, Phase modulation, Monochromatic aberrations, Actuators, Phase measurement, Aberration correction

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SPIE Journal Paper | 1 September 2010 Open Access

Open Access

Multiphoton microscopy of engineered dermal substitutes: assessment of 3-D collagen matrix remodeling induced by fibroblast contraction

Ana-Maria Pena, Dominique Fagot, Christian Olive, Jean-François Michelet, Jean-Baptiste Galey, Frederic Leroy, Emmanuel Beaurepaire, Jean-Louis Martin, Anne Colonna, Marie-Claire Schanne-Klein

JBO, Vol. 15, Issue 05, 056018, (September 2010) https://doi.org/10.1117/12.10.1117/1.3503411

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Proceedings Article | 9 May 2008 Paper

Paper

Contrast mechanisms and signal epidetection in THG microscopy of scattering tissues

Nicolas Olivier, Delphine Débarre, Emmanuel Beaurepaire

Proceedings Volume 6991, 699114 (2008) https://doi.org/10.1117/12.783376

KEYWORDS: Scattering, Tissues, Light scattering, Objectives, Microscopy, Interfaces, Monte Carlo methods, Dielectrics, Absorption, Gold

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Proceedings Article | 7 February 2007 Paper

Paper

Nonlinear microscopy of collagen fibers

M. Strupler, A.-M. Pena, M. Hernest, P.-L. Tharaux, A. Fabre, J. Marchal-Somme, B. Crestani, D. Débarre, J.-L. Martin, E. Beaurepaire, M.-C. Schanne-Klein

Proceedings Volume 6470, 647005 (2007) https://doi.org/10.1117/12.713576

KEYWORDS: Second-harmonic generation, Collagen, Tissues, Microscopy, Optical fibers, Visualization, Lung, Harmonic generation, Luminescence, Image processing

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Proceedings Article | 23 February 2006 Paper

Paper

Structure sensitivity and sources of contrast in third-harmonic generation (THG) microscopy of cells and tissues

D. Débarre, W. Supatto, E. Beaurepaire

Proceedings Volume 6089, 60891H (2006) https://doi.org/10.1117/12.645819

KEYWORDS: Microscopy, Tissues, Interfaces, Signal detection, Third-harmonic generation, Multiphoton microscopy, Visualization, Luminescence, Refractive index, Liver

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Proceedings Article | 22 February 2006 Paper

Paper

Spectroscopic analysis of skin intrinsic signals for multiphoton microscopy

Ana-Maria Pena, Mathias Strupler, Thierry Boulesteix, Karim Senni, Gaston Godeau, Emmanuel Beaurepaire, Marie-Claire Schanne-Klein

Proceedings Volume 6078, 607801 (2006) https://doi.org/10.1117/12.644777

KEYWORDS: Collagen, Second-harmonic generation, Skin, Luminescence, Biopsy, Molecules, Spectroscopy, Tissues, Multiphoton microscopy, Signal detection

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Proceedings Article | 22 February 2006 Paper

Paper

An all-optical approach to modulate and quantitatively analyse embryo morphogenetic movements by using ultrashort laser pulses

W. Supatto, D. Débarre, B. Moulia, E. Farge, E. Beaurepaire

Proceedings Volume 6088, 60881D (2006) https://doi.org/10.1117/12.645897

KEYWORDS: Microscopy, Modulation, Laser ablation, Tissues, Femtosecond phenomena, Motion analysis, Image analysis, Signal detection, Scattering, In vivo imaging

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Proceedings Article | 28 April 2005 Paper

Paper

Functionalized luminescent oxide nanoparticles for sodium channel imaging at the single molecule level

E. Beaurepaire, D. Giaume, V. Buissette, M.-P. Sauviat, D. Casanova, E. Auksorius, J.-L. Martin, K. Lahlil, T. Gacoin, J.-P. Boilot, A. Alexandrou

Proceedings Volume 5705, (2005) https://doi.org/10.1117/12.590737

KEYWORDS: Nanoparticles, Sodium, Luminescence, Europium, Ions, Oxides, Silicon, Microscopes, Absorption, Molecules

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Proceedings Article | 30 March 2005 Paper

Paper

In vivo analysis of Drosophila embryo developmental dynamics by femtosecond pulse-induced ablation and multimodal nonlinear microscopy

Willy Supatto, Delphine Debarre, Bruno Moulia, Eric Brouzes, Jean-Louis Martin, Marie-Claire Schanne-Klein, Emmanuel Farge, Emmanuel Beaurepaire

Proceedings Volume 5700, (2005) https://doi.org/10.1117/12.589425

KEYWORDS: Microscopy, Tissues, In vivo imaging, Femtosecond phenomena, Scattering, Surface plasmons, Multiphoton microscopy, Motion analysis, Luminescence, Modulation

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Proceedings Article | 29 March 2005 Paper

Paper

Multiphoton microscopy using intrinsic signals for pharmacological studies in unstained cardiac and vascular tissue

Emmanuel Beaurepaire, Thierry Boulesteix, Ana-Maria Pena, Nicole Pages, Karim Senni, Gaston Godeau, Martin-Pierre Sauviat, Marie-Claire Schanne-Klein

Proceedings Volume 5699, (2005) https://doi.org/10.1117/12.589558

KEYWORDS: Second-harmonic generation, Multiphoton microscopy, Arteries, Tissues, Microscopy, Collagen, Head, Luminescence, Harmonic generation, Visualization

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Proceedings Article | 8 September 2004 Paper

Paper

In vivo microdissection and live embryo imaging by two-photon microscopy to study Drosophila melanogaster early development

Willy Supatto, Eric Brouzes, Emmanuel Farge, Emmanuel Beaurepaire

Proceedings Volume 5463, (2004) https://doi.org/10.1117/12.545453

KEYWORDS: Tissues, Microscopy, Surface plasmons, In vivo imaging, Femtosecond phenomena, Luminescence, Green fluorescent protein, Scattering, Absorption, Natural surfaces

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Proceedings Article | 9 October 2003 Paper

Paper

Chiral chromophores for second harmonic microscopy

Marie-Claire Schanne-Klein, Thierry Boulesteix, Emmanuel Beaurepaire, Martin-Pierre Sauviat

Proceedings Volume 5139, (2003) https://doi.org/10.1117/12.499754

KEYWORDS: Second-harmonic generation, Molecules, Microscopy, Optical activity, Chromophores, Polarization, Nonlinear optics, Magnetism, Signal detection, Oscillators

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Proceedings Article | 2 November 2001 Paper

Paper

Strategies for improving depth-penetration of two-photon imaging in vivo

Martin Oheim, Emmanuel Beaurepaire, Jerome Mertz, Serge Charpak

Proceedings Volume 4431, (2001) https://doi.org/10.1117/12.447410

KEYWORDS: Luminescence, Objectives, Brain, Scattering, Tissues, In vivo imaging, Microscopes, Sensors, Laser scattering, Tissue optics

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Proceedings Article | 22 November 2000 Paper

Paper

Optical coherence microscopy for high-resolution biological imaging

Laurent Vabre, Arnaud Dubois, Emmanuel Beaurepaire, Albert Boccara

Proceedings Volume 4160, (2000) https://doi.org/10.1117/12.407632

KEYWORDS: Microscopes, Objectives, Tissues, Optical coherence tomography, Modulation, Image resolution, Tomography, Light emitting diodes, Optical coherence microscopy, CCD cameras

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Proceedings Article | 19 July 1999 Paper

Paper

Imaging through scattering media using cameras and parallel processing

Sandrine Leveque, Arnaud Dubois, Emmanuel Beaurepaire, Loic Pottier, Albert Boccara, Martial Lebec, Herve Saint-Jalmes

Proceedings Volume 3749, (1999) https://doi.org/10.1117/12.354984

KEYWORDS: Photons, Scattering media, Parallel processing, Sensors, Signal to noise ratio, Biomedical optics, Modulation, Cameras, Signal processing, Tissues

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Proceedings Article | 15 July 1999 Paper

Paper

3D imaging in biological tissues by tagging photon paths with ultrasound

Sandrine Leveque, Albert Boccara, Emmanuel Beaurepaire, Arnaud Dubois, Martial Lebec, Herve Saint-Jalmes

Proceedings Volume 3597, (1999) https://doi.org/10.1117/12.356827

KEYWORDS: Modulation, Speckle, Signal detection, Ultrasonics, CCD cameras, Tissues, Transducers, CCD image sensors, Ultrasonography, Signal to noise ratio

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Proceedings Article | 6 May 1999 Paper

Paper

High-resolution real-time full-field interference microscopy

Arnaud Dubois, Martial Lebec, Emmanuel Beaurepaire, Sandrine Leveque, Albert Boccara

Proceedings Volume 3605, (1999) https://doi.org/10.1117/12.347563

KEYWORDS: Microscopes, Objectives, Lenses, Light emitting diodes, Integrated circuits, Autoregressive models, Microscopy, 3D image processing, Mirrors, Silicon

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Proceedings Article | 16 April 1998 Paper

Paper

Optical coherence microscopy for the in-depth study of biological structures: system based on a parallel detection scheme

Emmanuel Beaurepaire, Philippe Gleyzes, Martial Lebec, Loic Blanchot, Herve Saint-Jalmes, Albert Boccara

Proceedings Volume 3250, (1998) https://doi.org/10.1117/12.305380

KEYWORDS: Modulation, Optical coherence tomography, Signal detection, Cameras, Objectives, Mirrors, Image resolution, Natural surfaces, CCD cameras, Phase shifts

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Proceedings Article | 1 January 1998 Paper

Paper

Low-coherence in-depth microscopy for biological tissue imaging: design of a real-time control system

Loic Blanchot, Martial Lebec, Emmanuel Beaurepaire, Philippe Gleyzes, Albert Boccara, Herve Saint-Jalmes

Proceedings Volume 3194, (1998) https://doi.org/10.1117/12.301057

KEYWORDS: Control systems design, Imaging systems, Microscopy, Tissues, Signal processing, CCD cameras, Computing systems, Multiplexing, Cameras, Signal detection

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Showing 5 of 33 publications

Proceedings Volume Editor (7)

SPIE Conference Volume | 30 August 2023

Advances in Microscopic Imaging IV

SPIE Conference Volume | 7 December 2021

Advances in Microscopic Imaging III

SPIE Conference Volume | 4 October 2019

Advances in Microscopic Imaging II

SPIE Conference Volume | 19 September 2017

Advances in Microscopic Imaging

SPIE Conference Volume | 4 August 2015

Advanced Microscopy Techniques IV; and Neurophotonics II

Showing 5 of 7 publications

Conference Committee Involvement (8)

Advances in Microscopic Imaging

28 June 2023 | Munich, Germany

Advances in Microscopic Imaging

20 June 2021 | Online Only, Germany

Advances in Microscopic Imaging

26 June 2019 | Munich, Germany

Advances in Microscopic Imaging

25 June 2017 | Munich, Germany

Advanced Microscopy Techniques IV

24 June 2015 | Munich, Germany

Showing 5 of 8 Conference Committees

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