Here, the structures fabricated using direct laser writing and interference lithography techniques are demonstrated. By the
tightly focused femtosecond laser beam in the thin gold film, various structures such as the gold bumps, needles, or cones
can be fabricated. The shape of the gold structures depends on the used laser pulse energy. By the treatment of the thin
films using nanosecond laser pulses, the direct synthesis of nanoparticles on the glass substrate can be realized. The laser
processing of photopolymers enables the formation of structures suitable for micro-optics.
The goal of this study is development of ultra-sensitive and reproducible SERS platform based on novel magnetoplasmonic nanoparticles produced by laser ablation. The magnetic part of hybrid nanoparticles ensures manipulation of the nanoparticles by magnetic field by arranging them at biological surfaces in a special geometry resulting in high and reproducible SERS. Magneto-plasmonic Au-Fe nanoparticles in colloidal suspension were prepared by picosecond laser ablation of evaporated iron and gold films on glass. The nanoparticles were characterized by UV-visible extinction, high resolution electronic microscopy, and Raman spectroscopy. EDX analysis revealed that the shell of nanoparticles (2−20 nm) consist of iron and the core is composed mostly of gold. The plasmonic behavior of nanoparticles was accessed by analysis of SERS spectra from adsorbed adenine as probe ligand. The fabrication of hybrid nanoparticles by laser ablation offers a new possibility for construction of SERS substrates with tunable optical and magnetic properties for biomedical sensing.
Fabrication of scaffolds for cell growth with appropriate mechanical characteristics is top-most important for successful
creation of tissue. Due to ability of fast fabrication of periodic structures with a different period, the holographic
lithography technique is a suitable tool for scaffolds fabrication. The scaffolds fabricated by holographic lithography can
be used in various biomedical investigations such as the cellular adhesion, proliferation and viability. These
investigations allow selection of the suitable material and geometry of scaffolds which can be used in creation of tissue.
Scaffolds fabricated from di-acrylated poly(ethylene glycol) (PEG-DA-258) over a large area by holographic lithography
technique are presented in this paper. The PEG-DA scaffolds fabricated by holographic lithography showed good
cytocompatibility for rabbit myogenic stem cells. It was observed that adult rabbit muscle-derived myogenic stem cells
grew onto PEG-DA scaffolds. They were attached to the pillars and formed cell-cell interactions. It demonstrates that the
fabricated structures have potential to be an interconnection channel network for cell-to-cell interactions, flow transport
of nutrients and metabolic waste as well as vascular capillary ingrowth. These results are encouraging for further
development of holographic lithography by improving its efficiency for microstructuring three-dimensional scaffolds out
of biodegradable hydrogels
In this work we present the latest results in the application of multi-photon polymerization for tissue engineering,
by fabricating microstructured artificial 3D scaffolds for stem cell growth. Microstructuring of large scale 3D
scaffolds is investigated and the direct laser writing technique is supplemented by fabrication by multi-beam
interference and micromolding of large scale structures. Within the limitation of our study, we conclude that the
proposed nonlinear direct laser writing technique offers rapid and flexible fabrication of biomedical components
with required shape, pore size and general porosity. The applications could target biostable and biodegradable
implants applied for bone or tissue replacement as well as drug delivery or release agents.
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