Dr. Evgeny A. Shirshin Profile

Dr. Evgeny A. Shirshin

at M V Lomonosov Moscow State Univ

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Publications (8)

SPIE Journal Paper | 30 September 2024 Open Access

Simultaneous assessment of NAD(P)H and flavins with multispectral fluorescence lifetime imaging microscopy at a single excitation wavelength of 750 nm

Boris Yakimov, Anastasia Komarova, Elena Nikonova, Artem Mozherov, Liubov Shimolina, Marina Shirmanova, Wolfgang Becker, Evgeny Shirshin, Vladislav Shcheslavskiy

JBO, Vol. 29, Issue 10, 106501, (September 2024) https://doi.org/10.1117/12.10.1117/1.JBO.29.10.106501

KEYWORDS: Fluorescence, Fluorescence lifetime imaging, Nicotinamide adenine dinucleotide, Emission wavelengths, Mode conditioning cables, Sensors, Fluorescence intensity, Cancer, Microscopy, Tumors

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SignificanceAutofluorescence characteristics of the reduced nicotinamide adenine dinucleotide and oxidized flavin cofactors are important for the evaluation of the metabolic status of the cells. The approaches that involve a detailed analysis of both spectral and time characteristics of the autofluorescence signals may provide additional insights into the biochemical processes in the cells and biological tissues and facilitate the transition of spectral fluorescence lifetime imaging into clinical applications.AimWe present the experiments on multispectral fluorescence lifetime imaging with a detailed analysis of the fluorescence decays and spectral profiles of the reduced nicotinamide adenine dinucleotide and oxidized flavin under a single excitation wavelength aimed at understanding whether the use of multispectral detection is helpful for metabolic imaging of cancer cells.ApproachWe use two-photon spectral fluorescence lifetime imaging microscopy. Starting from model solutions, we switched to cell cultures treated by metabolic inhibitors and then studied the metabolism of cells within tumor spheroids.ResultsThe use of a multispectral detector in combination with an excitation at a single wavelength of 750 nm allows the identification of fluorescence signals from three components: free and bound NAD(P)H, and flavins based on the global fitting procedure. Multispectral data make it possible to assess not only the lifetime but also the spectral shifts of emission of flavins caused by chemical perturbations. Altogether, the informative parameters of the developed approach are the ratio of free and bound NAD(P)H amplitudes, the decay time of bound NAD(P)H, the amplitude of flavin fluorescence signal, the fluorescence decay time of flavins, and the spectral shift of the emission signal of flavins. Hence, with multispectral fluorescence lifetime imaging, we get five independent parameters, of which three are related to flavins.ConclusionsThe approach to probe the metabolic state of cells in culture and spheroids using excitation at a single wavelength of 750 nm and a fluorescence time-resolved spectral detection with the consequent global analysis of the data not only simplifies image acquisition protocol but also allows to disentangle the impacts of free and bound NAD(P)H, and flavin components evaluate changes in their fluorescence parameters (emission spectra and fluorescence lifetime) upon treating cells with metabolic inhibitors and sense metabolic heterogeneity within 3D tumor spheroids.

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SPIE Journal Paper | 13 May 2023 Open Access

Monitoring the skin structure during edema in vivo with spatially resolved diffuse reflectance spectroscopy

Denis Davydov, Gleb Budylin, Alexey Baev, Daniil Vaypan, Elena Seredenina, Simon Matskeplishvili, Stanislav Evlashin, Armais Kamalov, Evgeny Shirshin

JBO, Vol. 28, Issue 05, 057002, (May 2023) https://doi.org/10.1117/12.10.1117/1.JBO.28.5.057002

KEYWORDS: Skin, Absorption, Water content, In vivo imaging, Diffuse reflectance spectroscopy, Monte Carlo methods, Structural monitoring, Data modeling, Linear regression, Scattering

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Proceedings Article | 5 March 2021 Poster

Optoacoustic staging, monitoring, and imaging of hematomas

Sergei Perkov, Oleg Grishin, Rinat Esenaliev, Simon Powell, Evgeny Shirshin, Vasiliy Chernyshev, Dmitry Gorin

Proceedings Volume 11642, 116423Z (2021) https://doi.org/10.1117/12.2584189

KEYWORDS: Optoacoustics, Tissues, Skin, Neuroimaging, Brain, Absorption

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Proceedings Article | 1 April 2020 Presentation

Fiber photonics solutions for tissue analysis for fast biomedical diagnostics (Conference Presentation)

Olga Bibikova, Alena Sergeeva, Stanislav Perevoschikov, Raushania Gaynullina, Iskander Usenov, Valeria Belikova, Anastasya Melenteva, Alexey Bocharnikov, Tatiana Sakharova, Evgeny Shirshin, Alexander Tonevitsky, Viacheslav Artyushenko

Proceedings Volume 11359, 113590D (2020) https://doi.org/10.1117/12.2555420

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Proceedings Article | 21 April 2016 Paper

Optical researches for cyanobacteria bloom monitoring in Curonian Lagoon

Evgeny Shirshin, Gleb Budylin, Boris Yakimov, Olga Voloshina, Genrik Karabashev, Marina Evdoshenko, Victor Fadeev

Proceedings Volume 9917, 99170V (2016) https://doi.org/10.1117/12.2239600

KEYWORDS: Remote sensing, Absorption, Hyperspectral sensing, Remote sensing radiometers, Water, Reflectivity, Satellites, Ecosystems, Organisms, Satellite imaging, Ocean optics, Luminescence

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Proceedings Article | 21 April 2016 Paper

SDS-binding assay based on tyrosine fluorescence as a tool to determine binding properties of human serum albumin in blood plasma

Nadezda Zhdanova, Evgeny Shirshin, Victor Fadeev, Alexander Priezzhev

Proceedings Volume 9917, 991713 (2016) https://doi.org/10.1117/12.2229850

KEYWORDS: Luminescence, Blood, Plasma, Fluorescence spectroscopy, Proteins, Molecules, Sodium, Data modeling, Diagnostics, Molecular spectroscopy, Absorption

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SPIE Journal Paper | 18 February 2015 Open Access

Native fluorescence spectroscopy of blood plasma of rats with experimental diabetes: identifying fingerprints of glucose-related metabolic pathways

Evgeny Shirshin, Olga Cherkasova, Tatiana Tikhonova, Elena Berlovskaya, Alexander Priezzhev, Victor Fadeev

JBO, Vol. 20, Issue 05, 051033, (February 2015) https://doi.org/10.1117/12.10.1117/1.JBO.20.5.051033

KEYWORDS: Luminescence, Blood, Plasma, Proteins, Glucose, Fluorescence spectroscopy, Plasma spectroscopy, Tissues, Cancer, Statistical analysis

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Proceedings Article | 27 July 2007 Paper

Non-linear fluorimetry of fluorescent proteins

A. Banishev, E. Shirshin, V. Fadeev

Proceedings Volume 6733, 67331B (2007) https://doi.org/10.1117/12.753276

KEYWORDS: Luminescence, Fluorescent proteins, Absorption, Chromophores, Proteins, Quantum efficiency, Molecules, Raman scattering, Remote sensing, Process modeling

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