Stimulated Raman scattering (SRS) microscopy is a well-established non-linear optical technique for label-free identification of biochemical components in cells and tissues. In this contribution, we present broadband SRS microscopy with differential multichannel-lock in detection
Stimulated Raman scattering (SRS) microscopy is a powerful technique for label-free identification of molecules based on their intrinsic vibrational spectrum. We present a novel approach to broadband SRS microscopy based on a recently developed balanced low-noise integrated-circuit multichannel lock-in amplifier with 10-μs integration time and 32 channels working in parallel, enabling multiplex SRS detection at speed faster than one hyperspectral frame per second. The system is powered by a narrowband Stokes pulse at 1040 nm and a broadband (approx. 500 cm^-1) pump pulse generated by a home-built low-noise optical parametric oscillator with up to 100-mW average power, covering the whole C-H stretching band. We measure stimulated Raman loss on the broadband pump pulse employing an in-line balanced detection approach to suppress the laser fluctuations and achieve close to shot-noise-limited sensitivity. We will show the system performances in high-resolution cell imaging.
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