Mr. Jeonghwan Son Profile

Jeonghwan Son

at Emory Univ

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Publications (3)

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Proceedings Article | 2 March 2022 Presentation + Paper

Resolution doubling in optofluidics and sample-scanning fluorescence microscopy

Biagio Mandracchia, Kidan Tadesse, Jeonghwan Son, Shu Jia

Proceedings Volume 11967, 1196702 (2022) https://doi.org/10.1117/12.2608994

KEYWORDS: Optofluidics, Microscopy, Microfluidics, Super resolution, Image resolution, Microscopes, Scanners, Imaging systems, Confocal microscopy, Optical diagnostics

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A great interest in high-throughput fluorescence microscopy for biological and medical imaging has led to the flourishing of new imaging methods where the sample is quickly scanned through the optics. Optofluidic microscopes use fluids’ properties as an additional degree of freedom for optical detection and microfluidics to perform simple and low-cost object manipulation. Even though several devices have been optimized for fluorescence-based imaging, these systems can rarely resolve sub-micron details, posing a limit to the structures that can be studied. An exception is represented by systems developed for particle detection, which are capable to quantify protein expression and analyze small molecules even at nanoscale resolution. However, in this case, high resolution requires a low emitter density and it cannot be used to visualize densely packed structures such as membranes and organelles. Hence, we have developed a system for sub-diffractionlimited optofluidic scanning microscopy (OSM) that uses the optofluidics paradigm to extract the inherent super-resolution information of a confocal system. OSM uses the optofluidic flow scanning scheme and a multifocal illumination pattern to obtain resolution doubling with minimal system complexity. In addition, it does not require any mechanical part for the scanning, so that it can be readily adapted to different levels of integration from commercial microscopes to on-chip configurations. This makes our system the most viable configuration for super-resolution optofluidics, being both suitable for continuous flow scanning and compatible with on-chip configurations through the adoption of integrated optics, like custom micro-lenses or Fresnel zone plates. Finally, we demonstrate how the same concept can be adapted to digital slide scanners for super-resolution whole slide imaging.

Proceedings Article | 2 March 2022 Presentation + Paper

A compact open-top light-sheet microscope for Optofluidic imaging

Jeonghwan Son, Biagio Mandracchia, Shu Jia

Proceedings Volume 11966, 1196608 (2022) https://doi.org/10.1117/12.2607324

KEYWORDS: Imaging systems, Optofluidics, Microfluidics, Reconstruction algorithms, Microscopy, Microscopes, Image resolution

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The fluorescence Optofluidic imaging have recently transformed conventional optical investigations of biological systems by exploiting the synergic combination of recent microscopy and integrated chips technologies. The implementation of light-sheet microscopy on Optofluidic imaging has further pushed the systemic capabilities towards fast and 3D-volumetric imaging. Here, we propose a new compact open-top light-sheet microscope to extend the study of 3D-fluorescence Optofluidic imaging. The proposed portable architecture of the system and customized reconstruction algorithms enable providing high throughput and volumetric reconstruction capability with an isometric single-cell resolution. The experimental results demonstrate the applicability of open-top light-sheet configuration to single-cell imaging in a flow. We anticipate the system to offer promising applications in clinical settings where the portability and 3D-volumetric analysis are required for fast diagnostics.

Proceedings Article | 20 June 2021 Presentation + Paper

Sub-diffraction-limit optofluidic scanning microscopy

Biagio Mandracchia, Jeonghwan Son, Shu Jia

Proceedings Volume 11786, 117860G (2021) https://doi.org/10.1117/12.2593661

KEYWORDS: Optofluidics, Microscopy, Microfluidics, Super resolution, Microscopes, Confocal microscopy, Zone plates, Visualization, System integration, Proteins

Read Abstract +

Optofluidic microscopes use fluids’ properties as an additional degree of freedom for optical detection and microfluidics to perform simple and low-cost object manipulation. In particular, several devices have been optimized for fluorescencebased imaging. These are usually based on the adaptation of established microscopy tools to the microfluidic system with different levels of integration. However, these systems can rarely resolve sub-micron details, posing a limit to the structures that can be studied. An exception is represented by systems developed for particle detection, which are capable to quantify protein expression and analyze small molecules even at nanoscale resolution. However, in this case high resolution requires a low emitter density and it cannot be used to visualize densely packed structures such as membranes and organelles. Hence, we have developed a system for sub-diffraction-limited optofluidic scanning microscopy (OSM) that uses the optofluidics paradigm to extract the inherent super-resolution information of a confocal system. OSM uses the optofluidic flow scanning scheme and a multifocal illumination pattern to obtain resolution doubling with minimal system complexity. In addition, it does not require any mechanical part for the scanning, so that it can be readily adapted to different levels of integration from commercial microscopes to on-chip configurations. This makes our system the most viable configuration for super-resolution optofluidics, being both suitable for continuous flow scanning and compatible with on-chip configurations through the adoption of integrated optics.

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