Infrared neural stimulation (INS) is a label-free optical neurostimulation technique that offers increased spatial specificity over traditional neurostimulation techniques. Initial studies on calcium signaling in the rat cortex demonstrated a multipart response indicating INS induces signaling in both neurons and astrocytes. We further characterized this response in cortical astrocytes in vitro, showing that different INS pulse structures induce varying levels of calcium signaling in these cells. Here we evaluate the effects of varying laser pulse parameters in cultured cortical neurons. We hypothesize that reducing the pulse duration will lead to lower stimulation thresholds. We show that INS induces a calcium response in vitro cortical neurons dependent on the total radiation exposure and time, indicating that the peak power of the infrared pulse influences INS efficacy. In conclusion, INS efficacy in vitro depends on the laser pulse width parameters.
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