Skin necrosis may occur due to no blood circulation after skin grafting. In present, it may not be easy to make sure that the blood is normally circulated to the trans implanted skin flap. A real-time laser speckle imaging modality (LSIM) developed in our laboratory was utilized to evaluate early skin necrosis. Two experiments were performed: 1) in-vitro optical tissue phantom (OTP) experiment to quantitatively identify the feasibility of blood flow variation; 2) in-vivo animal skin flap surgery experiment to induce skin necrosis in mouse. In comparison of laser speckle images (spatial and temporal speckle contrast analysis) and color images after skin flap surgery, laser speckle image resulted in better efficiency in evaluating skin necrosis than the color image. In laser speckle images, the temporal contrast image was more efficient than spatial contrast image in evaluating skin necrosis. In conclusion, it may be useful employing the real-time LSIMin noninvasively evaluating blood flow variation in skin necrosis.
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