In vivo rat brain imaging was demonstrated using the FF OCM (full field optical coherence microscopy) with SMMF (short multimode fiber) of a diameter of 125 μm and a length of 7.33 mm. The spatial resolution was measured to be 2.0 μm. The influences of motions for OCM images and methods to detect motions were investigated using image processing. Measurements were combinations of 3D OCM imaging (M1) with measurement time 10min and en face imaging (M2) at fixed depth with 17.9 fps and the interval time of 1 min during 30 min. For Rat 1 measured point was at the prim somatosens cortex, and the insertion length (IL) was 3.2 mm, the motions were not remarkable. En face OCM images with the region of X 56.3 μm, Y 56.3 μm, and Z 110 μm were measured. For Rat 2 measured point was at med parietal association cortex, and IL was 3.1 mm. Difference images and those intensity profiles showed the lateral motion of 5 µm between two sequent en face OCM images. For both cases, 3D images similarly showed the spatial arrangement of nerve fibers. The feasibility that the motions of tissues could be detected under the SMMF using sequential difference images was shown. This might be applicable to detect the motions in deep areas in measurements such as the optical intrinsic imaging
In achieving minimally invasive accessibility to deeply located regions the size of the imaging probes is important. We demonstrated full-field optical coherence tomography (FF-OCM) using an ultrathin forward-imaging short multimode fiber (SMMF) probe of 50 μm core diameter, 125 μm diameter, and 7.4 mm length for optical communications. The axial resolution was measured to be 2.14 μm and the lateral resolution was also evaluated to be below 4.38 μm using a test pattern (TP). The spatial mode and polarization characteristics of SMMF were evaluated. Inserting SMMF to in vivo rat brain, 3D images were measured and 2D information of nerve fibers was obtained. The feasibility of an SMMF as an ultrathin forward-imaging probe in FF-OCM has been demonstrated.
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