Surgeries performed on human eye require a high level of dexterity. Accuracy of widely used ophthalmic stereomicroscopes can be affected by limited depth perception and precision reduced by physiological tremor. Robotic arm-assisted manipulation of surgical tools improves precision, while intraoperative Optical Coherence Tomography (OCT) provides depth information and improves accuracy. Our hardware and software solutions enable tracking the tool tip to ensure the displayed OCT cross-scans, rather than 3-D volumes, are always locked to the tools’ tip position parsed from the robotic arm subsystem. Tool tracking errors for lateral movement and rotation are discussed for various velocities of the tool.
In this study we demonstrated the effectiveness of dynamic light scattering (DLS) in enhancing the resolution of optical coherence tomography (OCT) images of static samples. By carefully analyzing the movement of particles within the sample, utilizing the autocorrelation function of the backscattered light and performing averaging of OCT images captured at different time points, we were able to effectively suppress spatial coherence and achieve improved transverse resolution in the images. Additionally, this technique holds the potential for providing valuable insights into the internal movements of biological samples, such as blood flow. To validate our method, we conducted experiments using an OCT system and introduced scatterers exhibiting random Brownian motion. The enhanced spatial resolution was clearly demonstrated through the visualization of cross-sections of bars and the analysis of B-scans. Our findings pave the way for further advancements in OCT imaging techniques and offer promising applications in the study of static biological samples.
Optical Coherence Tomography (OCT) is a well-established modality for structural and functional imaging of the biological samples. Conventional scanning OCT combines the low temporal coherence with confocal gating to reject multiply scattered light. However, OCT uses a spatially coherent light source, and thus, is susceptible to speckle noise, which reduces the transverse resolution. We use dynamic light scattering to improve the transverse resolution. The dynamic scattering particles induce speckles, that change over time due to particle displacement. By incoherently averaging OCT images acquired under different particle distributions, we effectively suppress the spatial coherence and improve transverse image resolution.
We introduce a phase information-based, free-of-optimization multiple spectrometers’ spectral alignment method. Our technique relies on the observation that the slope of the unwrapped phase of the interferometric signal changes linearly with the same gradient for all identical spectrometers. Our method shows the clear benefit of shorter computational time compared to previously proposed methods based on optimization algorithms. The approach presented here can be used with commercially available spectrometers in which access to optical elements is limited and physical alignment is impossible.
Caries of the interdental space are usually difficult to detect with conventional bitewing radiography. We have developed an interdental probe for polarization-sensitive optical coherence tomography (PSOCT) that can distinguish between healthy and carious tooth structures. The development is based on a fiber optic probe with a compact motor design that allows volumetric imaging. In a clinical study, demineralization was detected using proximal PSOCT scans of premolars and molars, and compared with blind diagnoses from four dentists. The results show that PSOCT can detect both, early demineralization and advanced proximal caries, some of which could not be diagnosed with bitewing radiography.
We present a microscope-integrated Optical Coherence Tomography (OCT) system for aiding ophthalmic surgical manoeuvers. It generates two B-scans dynamically locked on the tip of the surgical tool to provide relevant depth cues. The low-latency architecture of our tool-tracking engine provides a position update rate of 200 Hz in a bid to minimize instantaneous tool position tracking errors. Likewise, the B-scan update rate of 200 Hz helps reduce motion artefacts while allowing for high signal-to-noise ratio averaged OCT images displayed at 50 Hz.
The ability to perform multi-meridian, simultaneous OCT measurements of air-induced corneal deformation is expected to highly improve the accuracy of assessing corneal biomechanics. We propose a simplified method targeting 3-D deformation measurement that could be introduced to swept-source OCT systems. We utilize a spatial-depth-encoded multiplexing to provide a 9-spot measurement of the deformation. The method is promising for the assessment of corneal asymmetries and diagnosis of corneal pathologies such as keratoconus. We present in detail the system and key requirements to provide simultaneous 9-spot deformation measurement. Finally, results on porcine eyes ex vivo and human eye in vivo are presented.
Image artifacts due to the bulk motion of the sample are well known and described. Some methods of correction in Full-Filed Swept-Source OCT were presented. For the measurement of dynamic sample motion, the axial motion artifacts can significantly influence measured signals. Here, we investigate the axial shift phenomena for measurements with low cost, reduced speed swept laser. Simulation results have led us to the way of correction of the axial shift artifact by manipulation of the phase of OCT fringes. Results of disturbed and undisturbed measurement of vibrating speaker membrane or induced deformations of the porcine eye are presented.
Optical coherence tomography (OCT) is a well-established modality for structural and functional imaging of the biological samples. Conventional scanning OCT combines the low temporal coherence with confocal gating to reject multiply scattered light. However, OCT uses a spatially coherent light source, and thus, is susceptible to speckle noise, which reduces the transverse resolution. We use dynamic light scattering to improve the transverse resolution. The dynamic scattering particles induce speckles, that change over time due to particle displacement. By incoherently averaging OCT images acquired under different particle distributions, we effectively suppress the spatial coherence and improve transverse image resolution.
Significance: To advance our understanding of the contrast observed when imaging with polarization-sensitive optical coherence tomography (PS-OCT) and its correlation with oral cancerous pathologies, a detailed comparison with histology provided via ex vivo fixed tissue is required. The effects of tissue fixation, however, on such polarization-based contrast have not yet been investigated.
Aim: A study was performed to assess the impact of tissue fixation on depth-resolved (i.e., local) birefringence measured with PS-OCT.
Approach: A PS-OCT system based on depth-encoded polarization multiplexing and polarization-diverse detection was used to measure the Jones matrix of a sample. A wide variety of ex vivo samples were measured freshly after excision and 24 h after fixation, consistent with standard pathology. Some samples were also measured 48 h after fixation.
Results: The tissue fixation does not diminish the birefringence contrast. Statistically significant changes were observed in 11 out of 12 samples; these changes represented an increase in contrast, overall, by 11% on average.
Conclusions: We conclude that the fixed samples are suitable for studies seeking a deeper understanding of birefringence contrast in oral tissue pathology. The enhancement of contrast removes the need to image immediately postexcision and will facilitate future investigations with PS-OCT and other advanced polarization-sensitive microscopy methods, such as mapping of the local optic axis with PS-OCT and PS-optical coherence microscopy.
Air-puff induced corneal deformation imaging reveals information highlighting normal and pathological corneal response to a non-contact mechanical excitation. Here, we present a novel customized swept-source optical coherence tomography system coupled with a collinear air-puff excitation. We acquired unobstructed dynamic corneal deformation on multiple meridians with two custom scan patterns over a field of view of up to 15 mm x 15 mm and selected puff profiles at unprecedented scan rates, both ex vivo and in vivo. We show that our system can detect corneal deformation profiles and deformation asymmetries that are useful for corneal biomechanics diagnostics and pathology screening.
Tissue with fibrillar architecture, such as collagen or muscle fiber, exhibits birefringence. In addition to the scalar amount of birefringence, the orientation of the birefringence axis, i.e. the fast optic axis, provides important information on the physical orientation of the fibrillar tissue components. We have previously demonstrated local optic axis mapping using bench-top fiber-based polarization-sensitive optical coherence tomography (PS-OCT), by compensating for the transmission through fiber and system elements, imperfect system alignment, and preceding tissue layers. Using depth-multiplexed PS-OCT, the compensation considers both retardation and diattenuation and is applied in the wavenumber domain, preserving the full axial resolution of the system. Here, we extend our approach to catheter-based imaging. Analyzing a reflection signal from the distal tip of the optical probe, we decompose the recovered system transmission into a static component and a varying catheter transmission to accurately correct for the rotation-dependent transmission through the catheter. Catheter-based local optic axis mapping is validated with a custom-made birefringence phantom. Imaging ex-vivo human bronchus demonstrates the utility of reconstructing the local optic axis orientation to assess airway smooth muscle (ASM), which is oriented approximately orthogonal from the surrounding tissue, offering strong optic axis orientation contrast. Thickening and contraction of the ASM is considered a primary cause of breathing difficulties, and the capacity to clearly image the ASM could lead to an improved understanding of diseases such as asthma.
Non-Gaussian beams can provide extended depth of focus (DOF) at constant and potentially uncompromised transverse resolution, as well as a degree of self-reconstruction after beam shadowing, which may be present in tissue imaging. Hence such beams are being developed for imaging systems throughout many disciplines, including endoscopic imaging, where they hold great potential. General possibilities include up to more than 20-fold extension of DOF, tunable working distance, imaging around obstacles and integrated all-fiber designs.
In all-fiber based optical imaging systems; however, these advantages are limited by system design considerations. Trade-offs between miniaturization, extended DOF, SNR, and fiber availability arise, and estimating the effects of design modifications can be difficult and time consuming.
We model zero-order quasi-Bessel illumination and detection for a range of common probe and sample materials based on an analytic solution of the Fresnel diffraction integral and compare the results to Gaussian beams. We show that these beams, on scales that match optical fiber dimensions, generally have an upper limit for the spot size above which their distinct advantages over Gaussian beams fade. Similarly, we show the existence of a lower limit of practical performance of quasi-Bessel beams, where the imaging SNR penalty compared to a Gaussian beam becomes significant. Additionally to general theoretic considerations we discuss designs, modeling and characterization of all-fiber imaging probes.
This work provides an accessible overview for researchers to estimate what potential benefit non-Gaussian beams can introduce into their optical imaging system.
KEYWORDS: In vivo imaging, Cornea, Elastography, Optical coherence tomography, Coherence (optics), Tissues, Control systems, Modulation, Eye, Animal model studies
Biomechanical properties of the cornea play key role in accurate measurement of the intraocular pressure (IOP). The aim of this study is to assess the impact of IOP on corneal hysteresis in porcine (ex vivo) and human (in vivo) eyes using swept source optical coherence tomography combined with the air-puff system (air-puff SS-OCT).
We developed air-puff SS-OCT to assess rapid dynamics of porcine corneas during the air pulse application. Both tissue displacement x(t) and air stimulus F(t) are acquired simultaneously that enables generation of corneal hysteresis F(x), which is a direct signature of viscoelastic properties of the cornea. The hysteresis loop can be quantified by calculation the parameters including maximum apex displacement, central corneal thickness, hysteresis area, elastic moduli etc.
Firstly, the corneal response of 35 ex-vivo porcine eyes to the air puff is determined for IOP ranging from 5 to 35 mmHg. The IOP level is set by a custom pressure control system. The IOP causes highly correlated changes in the proposed parameters of the hysteresis curve. Secondly, we investigate the modification of corneal hysteresis in 30 human corneas in vivo. The IOP is modulated by installation of 0,2% brimonidine eye drops (Alphagan) decreasing the IOP. The IOP is measured with air-puff non-contact tonometer (Topcon) and Goldmann tonometer and compared with hystereses generated by air-puff SS-OCT.
To conclude, IOP generates changes of corneal viscoelasticity in ex vivo animal model and in vivo human eyes. Non-invasive character, micrometer resolution and fast acquisition make our approach attractive for in vivo studies.
Airway inhalation injuries are present in up to a third of all major burns patients and are the leading cause of mortality among this population. Understanding the mechanism of injury could minimise oedema (swelling) and airway damage. In this study, we present an anatomical OCT (aOCT) imaging system, based on a 1300-nm wavelength, high-speed, long-range MEMS-VCSEL swept laser source, for real-time volumetric imaging and assessment of inhalation injuries in airways up to 3 cm in diameter. A custom fibre-optic probe with GRIN lens and micro prism is inserted though the nasal passage. Airway cross-sectional images acquired are used to assess airway diameter and lumen area, as well as to visualize the airway in three dimensions. Preliminary scans of healthy human subjects are presented. The effect of the supine position and fluid intake on airway geometry is quantified to better understand how these factors may contribute to the treatment outcomes of burns patients.
Visualization of the airway smooth muscle (ASM) layer is crucial in understanding its role in normal and abnormal airway function. Increased thickness of the ASM layer is one of the pathological hallmarks of airway remodeling in asthma and chronic obstructive pulmonary disease (COPD). The thickness of the ASM layer cannot be measured using current imaging techniques such as CT. Recently, Adams et al. introduced a birefringence microscopy platform, based on polarization sensitive optical coherence tomography (OCT), that enabled identification of ASM by its optic axis orientation in humans and allowed investigation of ASM contractile force ex vivo. In this work, we implemented passive depth-encoded polarization multiplexing and polarization-diversity detection in a 1300-nm swept-source OCT system. Compared with the previous inter-A-line modulation, such a strategy offers a simpler, more noise-resistant measure of the full polarization response of the tissue captured from a single A-line. We also refined the reconstruction of the depth-resolved birefringence properties to obtain the local optic axis orientation, corrected for the effect of preceding tissue layers and system distortions. Human bronchial samples were measured ex vivo in benchtop configuration. ASM layers, featuring muscle bundles oriented roughly at 90 degrees to the long axis of the airway, were extracted from 3D volumes by careful mapping of depth-resolved optic-axis orientation. Comparison of OCT measurements with H&E stained histological sections was performed to assess the accuracy of ASM delineation.
A fibre-based full-range polarisation-sensitive optical coherence tomography system is developed to enable complete capture of the structural and birefringence properties of the anterior segment of the human eye in a single acquisition. The system uses a wavelength swept source centered at 1.3 μm, passively depth-encoded, orthogonal polarisation states in the illumination path and polarisation-diversity detection. Off-pivot galvanometer scanning is used to extend the imaging range and compensate for sensitivity drop-off. A Mueller matrix-based method is used to analyse data. We demonstrate the performance of the system and discuss issues relating to its optimisation.
Polarization-sensitive OCT (PS-OCT) has proven useful in determining the stress-induced birefringence of non-biological materials, but such utility in biological tissues subjected to stress has not been well studied yet. To study stress-induced birefringence of biological tissues, we use a swept-source PS-OCT system with passively depth-encoded, orthogonal polarization states in the illumination path and polarization-diversity detection and a Mueller formalism in post-processing. We present measurements of stress-induced changes in the birefringence of non-biological and biological samples that provide useful benchmarks in further assessing the utility of this approach.
Quantitative assessment of upper airway geometry using optical coherence tomography in burns patients could provide physicians with the information needed to make critical decisions. We have developed a high speed catheter based OCT system capable of real time imaging in airways up to 3cm in diameter. Preliminary scans of inhalation injured airways are presented to demonstrate the feasibility of aOCT as a diagnostic tool for assessing burns patients.
Label-free imaging of the blood and lymphatic vessel networks of the conjunctiva of the eye is important in assessing the drainage pathways affected by glaucoma. We utilize the characteristically low signal in optical coherence tomography (OCT) provided by such vessels in ex vivo tissue to characterize their morphology in two and three dimensions. We demonstrate this method on conjunctiva from six porcine eyes, showing the ready visualization of both vessel networks. Such ex vivo characterization is a necessary precursor for future in vivo studies directed towards improving glaucoma surgery.
Polarisation sensitive optical coherence tomography (PS-OCT) offers additional intrinsic contrast to probe differences between healthy tissue and cancer that are often barely visible due to limited scattering contrast in an OCT image. PS-OCT reconstructs tissue birefringence from phase-sensitive measurements of orthogonal polarisation components of backscattering. In material science, polarisation has been used to study stress distribution, including the birefringence induced by stress in an otherwise isotropic material. Similar effects in biological tissues have not been well studied yet; however, may have application to tissues subjected to stress, e.g., tendons, muscles, lens, cornea or airway smooth muscle (ASM). The objective of this work is to explore stress-induced birefringence in tissue. We employ an advanced swept source-based PS-OCT system capable of measurement of tissue local polarisation properties. The sample in both cases is illuminated with orthogonal, passively depth-encoded polarisation states. Light returning from the tissue is detected via a polarisation-diversity detection module and a Mueller formalism is used to reconstruct polarisation properties (including retardation, diattenuation, and depolarisation) of the tissue. In this study, we demonstrate the measurement of stress-induced birefringence in phantoms and in soft tissues with polarisation sensitive optical coherence tomography.
We have been developing an automated method to image lymphatic vessels both ex vivo and in vivo with optical coherence tomography (OCT), using their optical transparency. Our method compensates for the OCT signal attenuation for each A-scan in combination with the correction of the confocal function and sensitivity fall-off, enabling reliable thresholding of lymphatic vessels from the OCT scans. Morphological image processing with a segment-joining algorithm is also incorporated into the method to mitigate partial-volume artifacts, which are particularly evident with small lymphatic vessels. Our method is demonstrated for two different clinical application goals: the monitoring of conjunctival lymphatics for surgical guidance and assessment of glaucoma treatment; and the longitudinal monitoring of human burn scars undergoing laser ablation treatment. We present examples of OCT lymphangiography ex vivo on porcine conjunctivas and in vivo on human burn scars, showing the visualization of the lymphatic vessel network and their longitudinal changes due to treatment.
The mechanical forces that living cells experience represent an important framework in the determination of a range of intricate cellular functions and processes. Current insight into cell mechanics is typically provided by in vitro measurement systems; for example, atomic force microscopy (AFM) measurements are performed on cells in culture or, at best, on freshly excised tissue. Optical techniques, such as Brillouin microscopy and optical elastography, have been used for ex vivo and in situ imaging, recently achieving cellular-scale resolution. The utility of these techniques in cell mechanics lies in quick, three-dimensional and label-free mechanical imaging. Translation of these techniques toward minimally invasive in vivo imaging would provide unprecedented capabilities in tissue characterization. Here, we take the first steps along this path by incorporating a gradient-index micro-endoscope into an ultrahigh resolution optical elastography system. Using this endoscope, a lateral resolution of 2 µm is preserved over an extended depth-of-field of 80 µm, achieved by Bessel beam illumination. We demonstrate this combined system by imaging stiffness of a silicone phantom containing stiff inclusions and a freshly excised murine liver tissue. Additionally, we test this system on murine ribs in situ. We show that our approach can provide high quality extended depth-of-field images through an endoscope and has the potential to measure cell mechanics deep in tissue. Eventually, we believe this tool will be capable of studying biological processes and disease progression in vivo.
In this paper, we use swept source optical coherence tomography combined with air-puff module (air-puff SS-OCT) to
investigate the properties of the cornea. During OCT measurement the cornea was stimulated by short, air pulse, and
corneal response was recorded. In this preliminary study, the air-puff SS-OCT instrument was applied to measure behavior
of the porcine corneas under varied, well-controlled intraocular pressure conditions. Additionally, the biomechanical
response of the corneal tissue before, during and after crosslinking procedure (CXL) was assessed. Air-puff swept source
OCT is a promising tool to extract information about corneal behavior as well as to monitor and assess the effect of CXL.
Optical coherence elastography (OCE) is emerging as a potentially useful tool in the identification of a number of diseases. In our group, we are developing OCE techniques based on compressive loading. Typically, these techniques employ a quasi-static mechanical load introduced by uniaxially compressing a sample with a rigid plate. The resulting deformation of the sample is measured using phase-sensitive detection and the local axial strain is estimated from the slope of displacement over a finite depth in the sample, providing qualitative mechanical contrast. In this talk, an overview of our work will be given and some of the outstanding challenges described. Our group’s work in OCE can broadly be divided into four streams, each of which will be described in detail in the talk: system development; techniques; quantification; and applications.
• System development: The phase-sensitive OCE method we have developed will be described, as well as a high resolution optical coherence microscopy-based elastography system suitable for imaging cellular-scale mechanical properties.
• Techniques: In addition to presenting techniques to estimate strain, our approaches to imaging tissue viscoelasticity and nonlinearity will be described. A technique to segment elastograms based on strain heterogeneity will be presented.
• Quantification: Methods under development to quantify tissue stiffness in compression OCE will be described. This work is enabled by optical palpation and solutions to the forward and inverse elasticity problems.
• Applications: Three applications areas will be described: intraoperative assessment of tumour margins, mapping stiffness in tumour biology and assessing the stiffness of cardiovascular tissue in an animal model.
We present a comprehensive imaging methodology for 3D structural and functional measurements of fertilized mouse oocytes. In contrary to methods used for mouse zygote imaging so far OCT provides 3D data without z axis movement of sample or objective lens. Furthermore, complex scanning protocols used in this study give access to different scales of repetition times and thus may become a tool for investigation of a different dynamic processes. Additionally, proposed scanning approach via variety of statistic operations can be used to enhance the quality of structural images. OCT system capabilities are presented and compared to standard microscopy. With a single 3D measurements one can extract 3D structure of the oocytes as well as en-face images that correspond to both bright and dark field microscopy. As an example of dynamic oocyte imaging pronuclei motion during development is presented. Limitations and possibilities of the new system are discussed.
Availability of the long-depth-range OCT systems enables comprehensive structural imaging of the eye and extraction of biometric parameters characterizing the entire eye. Several approaches have been developed to perform OCT imaging with extended depth ranges. In particular, current SS-OCT technology seems to be suited to visualize both anterior and posterior eye in a single measurement. The aim of this study is to demonstrate integrated anterior segment and retinal SS-OCT imaging using a single instrument, in which the sample arm is equipped with the electrically tunable lens (ETL). ETL is composed of the optical liquid confined in the space by an elastic polymer membrane. The shape of the membrane, electrically controlled by a specific ring, defines the radius of curvature of the lens surface, thus it regulates the power of the lens. ETL can be also equipped with additional offset lens to adjust the tuning range of the optical power. We characterize the operation of the tunable lens using wavefront sensing. We develop the optimized optical set-up with two adaptive operational states of the ETL in order to focus the light either on the retina or on the anterior segment of the eye. We test the performance of the set-up by utilizing whole eye phantom as the object. Finally, we perform human eye in vivo imaging using the SS-OCT instrument with versatile imaging functionality that accounts for the optics of the eye and enables dynamic control of the optical beam focus.
A novel, time-resolved interferometric technique is presented allowing the reconstruction of the complex electric field output of a fast frequency swept laser in a single-shot measurement. The power of the technique is demonstrated by examining a short cavity swept source designed for optical coherence tomography applications, with a spectral bandwidth of 18 THz. This novel analysis of the complete electric field reveals the modal structure and modal evolution of the device as well as providing a time-resolved real-time characterization of the optical spectrum, linewidth and coherence properties of a dynamic rapidly swept laser.
Tunable semiconductor laser for 1025-1095 nm spectral range is developed based on the InGaAs semiconductor optical amplifier and a narrow band-pass acousto-optic tunable filter in a fiber ring cavity. Mode-hop-free sweeping with tuning speeds of up to 104 nm/s was demonstrated. Instantaneous linewidth is in the range of 0.06-0.15 nm, side-mode suppression is up to 50 dB and polarization extinction ratio exceeds 18 dB. Optical power in output single mode fiber reaches 20 mW. The laser was used in OCT system for imaging a contact lens immersed in a 0.5% intra-lipid solution. The cross-section image provided the imaging depth of more than 5mm.
We analyse the dynamical behaviour of a short cavity OCT swept-source laser experimentally and theoretically. Mode-hopping, sliding frequency mode-locking and chaos are all observed during the laser sweep period. Hetero- dyne measurements of laser dynamics allows some insight into the behaviour of the laser, while interferometric techniques allow the full phase reconstruction of the laser electric field. A delay differential equation enables modelling of the laser output, and laser parameters can be altered to provide optimisation conditions for future laser designs.
We show results of a project which focuses on detection of activity in neural tissue with Optical Coherence Tomography (OCT) methods. Experiments were performed in neural cords dissected from the American cockroach (Periplaneta americana L.). Functional OCT imaging was performed with ultrahigh resolution spectral / Fourier domain OCT system (axial resolution 2.5 μm). Electrical stimulation (voltage pulses) was applied to the sensory cercal nerve of the neural cord. Optical detection of functional activation of the sample was performed in the connective between the terminal abdominal ganglion and the fifth abdominal ganglion. Functional OCT data were collected over time with the OCT beam illuminating selected single point in the connectives (i.e. OCT M-scans were acquired). Phase changes of the OCT signal were analyzed to visualize occurrence of activation in the neural cord. Electrophysiology recordings (microelectrode method) were also performed as a reference method to demonstrate electrical response of the sample to stimulation.
None of currently used tonometers produce estimated IOP values that are free of errors. Measurement incredibility arises
from indirect measurement of corneal deformation and the fact that pressure calculations are based on population
averaged parameters of anterior segment. Reliable IOP values are crucial for understanding and monitoring of number of
eye pathologies e.g. glaucoma. We have combined high speed swept source OCT with air-puff chamber. System
provides direct measurement of deformation of cornea and anterior surface of the lens. This paper describes in details the
performance of air-puff ssOCT instrument. We present different approaches of data presentation and analysis. Changes
in deformation amplitude appears to be good indicator of IOP changes. However, it seems that in order to provide
accurate intraocular pressure values an additional information on corneal biomechanics is necessary. We believe that
such information could be extracted from data provided by air-puff ssOCT.
In this pilot study we demonstrate results of structural Fourier domain OCT imaging of the nervous system of
Periplaneta americana L. (American cockroach). The purpose of this research is to develop an OCT apparatus enabling
structural imaging of insect neural system. Secondary purpose of the presented research is to develop methods of the
sample preparation and handling during the OCT imaging experiments. We have performed imaging in the abdominal
nerve cord excised from the American cockroach. For this purpose we have developed a Fourier domain / spectral OCT
system operating at 820 nm wavelength range.
We have developed and applied a high-speed Spectral OCT system to image small animal brains. OCT imaging with
high spatial resolution and application of multi-parameter approach enabled cortical blood flow visualization. We
imaged the brain vascular network of an anesthetized mouse stroke model. We demonstrated the impact of induced
stroke on the brain vasculature. The preliminary studies have revealed local ischemia in the areas of the stroke.
KEYWORDS: Optical coherence tomography, Image segmentation, Cornea, Image resolution, Eye, 3D image processing, In vivo imaging, 3D acquisition, Data acquisition, Mode locking
We present applicability of the high speed swept-source optical coherence tomography for in vivo imaging of the anterior segment of the human eye. Three dimensional imaging of the cornea with reduced motion artifacts is possible by using swept source with Fourier domain mode locking operating at 200kHz with 1300nm central wavelength. High imaging speeds allow for assessment of anterior and posterior corneal topography and generation of thickness and elevation maps.
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