To collect the volumetric image of the spinal cord using optical coherence tomography, the traditional way is to successively repeat the process of cutting the sample and imaging the revealed part. This common method is quite lengthy and laborious. Here, we designed circular scanning optical coherence tomography that has a customized ring-shaped silver-coated mirror reflecting incoming light in a circular pattern around the spinal cord. The sample is carried through that mirror by the linear stage during image acquisition. The results suggest that our system is suitable for the study of the spinal cord as well as applicable to other bigger samples when adapting clearing methods.
Longitudinal imaging of live organisms is essential to life science research aiming to understand biological processes. Recently, the imaging system has been housed for longitudinal imaging by mounting the optical imaging system in an incubator, while facing the limitations of single-modality and low throughput. Here, we proposed the OCToScope, a multi-modal, high-throughput and compact platform that is compatible with commercial incubators. OCToScope incorporates Optical Coherence Tomography(OCT) to achieve non-invasive three-dimensional imaging, and fluorescent imaging that retrieves cell-specific information. OCToScope also supports automatic scanning of mass samples quantity with motorized x-y-z stage and perform high time-resolution imaging for long-term studies. we used OCToScope to provide a systematic and comprehensive characterization of human heart organoids for over 30 days without any interference.
we introduce automated serial OCM toward statistical 3D digital histopathology. Our research is the extension of previous work in order to enhance the process of imaging acquisition. Our approach has three unique features, (1) surface tracking, (2) single body and automated system combined vibratome and microscopic imaging head, and (3) selection of magnification. In validation test, various mouse organs were imaged and quantified at the region of interest which presented less labor and shorten image acquisition time compared to previous works.
Optical coherence tomography (OCT) is a high-resolution and non-invasive internal structural imaging technique. Since the first introduction of OCT, it has been widely studied to enhance the scanning speed of the system to enhance the applicability. Spectral-domain OCT (SD-OCT) is one of the representative types of Fourier-domain OCT, which consisted with lower prices than swept-source OCT and offers higher axial resolution, but there are limited hardware performance to improve the scanning speed. In this paper, we introduced the space-time division multiplexing (STDM) method-based superfast SD-OCT with 1 MHz A-scan rate. In terms of the time-division method, dual-cameras were implemented in a single spectrometer to reduce the alignment error between each camera and fully utilize the operating time of camera by remove the dead time. In addition, the path length difference of the two-sample arm is accurately controlled to utilize the space-division method. By concurrently integrating the time- and space-division methods in STDM with GPU parallel computing, 32 volume/sec was acquired. The quantitative evaluation of the performance of STDM-OCT was analyzed with sensitivity roll-off and image quality comparison measured at different depth. The proposed STDM-OCT is able to enlarge the application of OCT including biomedical research areas, which require a high-speed scanning system.
Optical imaging techniques with physical tissue sectioning have become indispensable tools. However, acquiring volumetric anatomy of multiple organs and statistical studies remain a difficult challenge due to light scattering and long data acquisition period. Here, we propose a novel protocol for the high-throughput and quantitative analysis of 3D mouse organs using Coherence gating imaging (CGI) with tissue clearing. For statistical analysis, we also applied deep learning and outcomes were compared with computed tomography. Our preliminary results can improve imaging depth as well corresponding acquisition time, which would be promising tool for 3D digital histopathology.
KEYWORDS: Optical coherence tomography, In vivo imaging, Sodium, Microscopes, Scanning electron microscopy, Magnetic resonance imaging, Real time imaging, Image resolution, Agriculture, Imaging systems
In this study, Optical coherence tomography (OCT) is demonstrated as a plausible optical tool for in vivo detection of plant seeds and its morphological changes during growth. The experiment was carried out on Capsicum annuum seeds that were treated with different molar concentrations of NaCl to investigate the most optimal concentration for the seed growth. The monitoring process was carried out for 9 consecutive days. The in vivo 2D OCT images of the treated seeds were obtained and compared with seeds that were grown with sterile distilled water. The obtained results confirm the feasibility of using OCT for the proposed application. Normalized A-scan analysis method is utilized for supporting the concluded results.
A method for depth enhancement is presented using a bidirectional imaging modality for spectral domain optical coherence tomography (SD-OCT). Two precisely aligned sample arms along with two reference arms were utilized in the optical configuration to scan the samples. Using exemplary images of the optical resolution target, Scotch tape, a silicon sheet with two needles, and a leaf, we demonstrated how the developed bidirectional SD-OCT imaging method increases the ability to characterize depth-enhanced images. The results of the developed system were validated by comparing the images with the standard OCT configuration (single-sample arm setup). Given the advantages of higher resolution and the ability to visualize deep morphological structures, this method can be utilized to increase the depth dependent fall-off in samples with limited thickness. Thus, the proposed bidirectional imaging modality is apt for cross-sectional imaging of entire samples, which has the potential capability to improve the diagnostic ability.
We conducted an initial feasibility study using real-time magneto-motive optical Doppler tomography (MM-ODT) with enhanced contrast to investigate the detection of superparamagnetic iron oxide (SPIO) magnetic nanoparticles implanted into in vivo melanoma tissue. The MM-ODT signals were detected owing to the phase shift of the implanted magnetic nanoparticles, which occurred due to the action of an applied magnetic field. An amplifier circuit-based solenoid was utilized for generating high-intensity oscillating magnetic fields. The MM-ODT system was confirmed as an effective in vivo imaging method for detecting melanoma tissue, with the performance comparable to those of conventional optical coherence tomography and optical Doppler tomography methods. Moreover, the optimal values of the SPIO nanoparticles concentration and solenoid voltage for obtaining the uppermost Doppler velocity were derived as well. To improve the signal processing speed for real-time imaging, we adopted multithread programming techniques and optimized the signal path. The results suggest that this imaging modality can be used as a powerful tool to identify the intracellular and extracellular SPIO nanoparticles in melanoma tissues in vivo.
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