This study aims towards applying the intrinsic fluorescence technique, extracted from polarized fluorescence, to
detect subtle biochemical changes occurring during the progression of cancer from human cervical tissue samples. The
efficacy of this technique, earlier validated through tissue phantoms, is tested in human cervical tissues by comparing the
biochemical changes for diagnostic purpose at different wavelengths. It is pertinent to note that the co and crosspolarized
fluorescence do not display the high sensitivity obtained through extracted intrinsic fluorescence. We observed
that sensitivity and specificity of intrinsic fluorescence technique is high at 325 and 370nm for Collagen and NADH
respectively in comparison to 350nm excitation wavelength. It may be concluded that decoupled information at 325 and
370nm wavelengths for collagen and NADH respectively, through intrinsic fluorescence provides better diagnostic
parameter for early detection of cervical dysplasia. This information can provide a guiding path for designing a probe for
clinical purpose.
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