There is widespread concern about the safety of COVID-19 vaccinations related to platelet hyperactivity. However, their long-term influence on platelet activity remains unknown. We address this issue by applying a high-speed bright-field microscope based on optical frequency-division multiplexing and microfluidics for massive image-based analysis. We performed image-based single-cell profiling and temporal monitoring of circulating platelet aggregates in the blood samples of healthy human participants before and after they received three vaccination doses over a nearly one-year period. The results demonstrate no significant or persistent change in platelet activity after vaccine doses.
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