Analysis of multiple interactions of probe light with moving scattering particles indicates the manner in which scattering occurs. The study of statistical properties of Doppler shifted components of scattered light allows one to diagnose the structure and dynamic properties of scattering medium. In this paper, we consider an influence of the statistical properties of pathlength distributions that characterize the probe light transfer in scattering medium on analyzed dynamic speckle patterns. The convenient way to analyze dynamic speckle and to obtain in such manner the information about dynamic properties of scattering medium is to apply the contrast analysis of time-averaged speckle images known as Laser Speckle Contrast Analysis (LASCA) technique. Possibility to analyze the non-stationary layered media with use of the LASCA technique is discussed. Some manifestations of the statistical properties of the pathlength distributions of scattered field partial components in Doppler shift frequency distributions for these components are studied with use of the Monte-Carlo simulation.
Statistical analysis of images of time-integrated dynamic speckle patterns is considered as the tool for diagnostics and imaging of in vivo tissue dynamics such as blood microcirculation in superficial layers of human tissues and organs. Basic approach for blood microcirculation monitoring using the contrast analysis of time-averaged speckle images is known as LASCA (Laser Speckle Contrast Analysis) technique. This paper presents the modified version of LASCA, which is based on application of the localized probe light source and the spatial filtration of analyzed speckle pattern in the object plane. Being compared with classical LASCA technique, this method has the certain disadvantage as the necessity of scanning procedure to provide the reconstruction of maps of blood microcirculation parameters, but it gives the additional possibilities for the analysis of depth distributions of these parameters. Theoretical background for the depth-resolved analysis of blood microcirculation parameters on the basis of the concept of effective optical paths distributions for multiple scattered probe light is considered.
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