We demonstrate video-rate spontaneous Raman imaging by combining lightsheet microscopy that harnesses non-diffracting Airy beams to efficiently illuminate large specimen regions with image acquisition and reconstruction at the subphoton per pixel levels. We validated these benefits by imaging a wide variety of samples, including organic materials and the metabolic activity of single living yeast cells. Overall, our method not only enables video-rate imaging rates, but also requires 1000-fold less irradiance levels than state-of-the-art coherent Raman microscopy. As such, we expect this approach will greatly accelerate the reliability and reproducibility of Raman imaging in both fundamental research and clinical applications.
We report the integration of quantitative-phase imaging (QPI) with light-sheet (LS) fluorescent microscopy on to a standard inverted microscope that retains compatibility with microfluidics. QPI enables label-free imaging and number-density quantification of single cells and their organelles. Conversely, LS yields considerable speed and phototoxicity gains in quantifying the 4D dynamics of gene-encoded fluorescent biomarkers. We will detail the system design that relied on spatial light interferometry for QPI and an accelerating Airy-beam light-sheet for fluorescence, its performance, as well as results of a representative multivariate imaging analysis of single-cell metabolism.
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