A photofunctionalisable hydrogel based on copolymers of a nitroveratryl chloroformate derivative with acrylamide has been developed that permits the uncaging of amine groups on exposure to patterns of light at 405 nm using a watersoluble thioxanthone sensitizer. Hydrogel films of 3, 35 and 100 μm thickness were fabricated to use as leaky waveguide and grating sensors. The smallest feature sizes produced in 35 and 100 μm thick films were 21.6 and 43.2 μm respectively, limited by the depth of field of the digital projector. It was found that essentially complete deprotection could be obtained in ~2 minutes. The two thicker hydrogel films were used to create amplitude and phase gratings, while the thinnest film was used as a leaky waveguide to investigate the porosity of the films and monitor immobilization of protein using a PEG linker. The amplitude grating formed by reacting the uncaged amines with fluorescein isothiocyanate was shown to act as a pH sensor with resolution 0.0325 pH units. Protein immobilization using glutaraldehyde as a linker to create a phase grating was attempted, which gave a strong signal with glutaraldehyde but no subsequent signal with BSA. Investigation with a thin film acting as a leaky waveguide showed that glutaraldehyde significantly reduces the porosity of the film to high molecular mass species. Finally, leaky waveguiding was used to monitor protein immobilization using an amine-reactive PEG linker, showing that selective immobilization occurred in the exposed regions of the film without affecting the sensitivity of the film to bulk index changes.
Average iron content of ferritin has a potential to serve as a biomarker for early identification of high-risk trauma patients at point-of-care (PoC). Appropriate therapies can then be administered to reduce morbidity and mortality. Currently, protein and iron levels are measured separately using enzyme-linked immunosorbent assay (ELISA) and UV or atomic absorption spectroscopy (AAS) respectively, but the use of two completely different methods adds to the complexity and analysis time of the combined measurement. As a result, these methods are unsuitable for PoC analysis. To address this gap, we report a biosensor for measuring the average iron content of ferritin in a single step. The biosensor was based on a dye-doped leaky waveguide (LW), which operates in the entire visible wavelength range, and hence allowed the measurement of differences in the optical dispersion of ferritin and apoferritin to determine the average iron content of the protein. The LW biosensor comprised a 1.54 micron thick mesoporous chitosan slab waveguide with immobilized antibodies against ferritin/ apoferritin to measure the optical dispersion of 110 nM protein. Based on the baseline noise, the limit of detection for this method is ~700 pM for ferritin/ apoferritin. The biosensor has a significant potential for PoC measurement of the average iron content of serum ferritin and, in future, the total protein cencentration.
Previously, in high repetition rate femto second laser processing novel laser matter interacting effects were reported,
such as heat accumulation and particle shielding. In this study, high repetition rate laser processing was investigated to
discuss and understand the impact of laser repetition rate and accompanied accumulative laser material interacting
effects. Therefore, a high repetition rate femto second fibre laser setup joint together with galvo scanner technology was
applied in laser micro machining of metals (copper, stainless steel, aluminium). High repetition rate laser processing of
aluminium and stainless steel lead to considerably lowered ablation thresholds accompanied with higher ablation rates.
Laser ablation behaviour of copper was almost independent of the repetition rate with neither considerable lower ablation
thresholds nor higher ablation rates. For explanation, heat accumulation caused by higher repetition rates were assumed
as mainly ablation behaviour influencing effect, but thermal material properties have to be considered.
Furthermore laser machining examples demonstrate the possibilities and limits of high repetition rate laser processing in
3d micro structuring. Thus, by using innovative scanning systems and machining strategies very short processing times
were achieved, which lead to high machining throughputs and attract interest of the innovative laser technology in Rapid
Micro Tooling. For discussion, high repetition rate processing results are evaluated by means of comparative machining
examples obtained with 1 kHz femto second laser system.
A droplet-based microfluidic device has been developed for the controlled dilution and sorting of droplets by means of
electrokinetic forces. Neutral and cationic dyes have been tested in order to demonstrate the dilution efficiency. In
addition, yeast cells and latex beads were successfully enclosed in droplets in a controlled manner. Experiments results
demonstrate that even under rapid concentration changes, the rate of production and size of the droplets remained
constant. Following the generation of the diluted droplets, the remaining net surface charge allows them to be sorted
according to their dilution.
KEYWORDS: Sensors, Motion models, Target detection, Data modeling, Submerged target detection, Motion measurement, Detection and tracking algorithms, Time metrology, Signal to noise ratio, Monte Carlo methods
In this paper, we assess the capability of underwater hydrophone (UH) arrays to locate and track manoeuvring targets. A UH array is a horizontal line array of omnidirectional pressure sensors that is deployed on the seabed. The measurements at each UH array are then affected by two idiosyncrasies, termed "source direction ambiguity" and "coning error". In this paper, the posterior Cramer-Rao lower bound(PCRLB) is used as the measure of system performance, providing a bound on the optimal achievable accuracy of target state estimation. We demonstrate the impact of the measurement idiosyncrasies on the PCRLB, with the bound shown to be greater (poorer performance) than when using standard bearings-only sensors. We also include clutter (i.e. we allow each measurement to be either target generated or a false positive), as well as both state-dependent measurement errors and a state-dependent probability of detection. Building on previous work, we show that the measurement origin uncertainty can again be expressed as an information reduction factor (IRF), with this IRF now shown to be a function of both the target range and orientation in relation to each UH array. We consider simulated scenarios that contain features characteristic of recent sea trials conducted by QinetiQ Ltd. The two key features of the trial scenarios is that we have very sparse prior knowledge, and each target has the potential to perform a series of manoeuvres. We use a recent PCRLB formulation for tracking manoeuvring targets that approximates the potentially multi-modal target distribution using a best-fitting Gaussian distribution. We present simulation results for multi-sensor scenarios, demonstrating that this is indeed a difficult tracking problem. Tracking is particularly difficult when the target crosses the line of the UH arrays, making triangulation difficult; and when the target is in the "end-fire" of at least one UH array. It is also difficult to detect and triangulate distant targets. Future work will investigate the tightness of the PCRLB when compared with the performance of state-of-the-art tracking algorithms.
The micromoulding of polymers has provided an ideal fabrication route for the construction of single-use integrated devices for electrokinetic microseparations. Our designs have incorporated injector geometry that allows variable injection volumes in microchannels controlled by a combination of programmed hydrodynamics and electrophoresis. The utility of the injection scheme is demonstrated for isotachophoresis separations. Further developments have lead to the incorporation of parallel opposed conductivity detection electrodes in two ways. Firstly, by the injection of conducting polymer into a pre-molded channel system and secondly through the molding of the polymer microchannels around electrodes pre-molded in conducting polymer. This has provided a potential rapid manufacturing route for low cost polymer separation devices. As well as integrated conductivity detection electrodes, simple optical elements have been incorporated into injection molded ITP devices to permit detection of the separated bands by optical means. A simple spherical lens was incorporated into the top section of the device which included the sample and buffer reservoirs. The lens was positioned directly above the conductivity detection electrodes, to permit simultaneous electrochemical and optical detection. The lens was used to perform visible absorption spectroscopy using an Ocean Optics spectrography and tungsten-halogen fiber-optic white light source.
Isotachophoresis is an electrokinetic separation technique in which a sample volume is placed between two electrolytes. The resulting separation consists of contiguous zones of constant composition whose length is proportional to component concentration in the sample. This paper describes the development of miniaturized formats consisting of microchannel structures fabricated in polymer substrates.
Access to the requested content is limited to institutions that have purchased or subscribe to SPIE eBooks.
You are receiving this notice because your organization may not have SPIE eBooks access.*
*Shibboleth/Open Athens users─please
sign in
to access your institution's subscriptions.
To obtain this item, you may purchase the complete book in print or electronic format on
SPIE.org.
INSTITUTIONAL Select your institution to access the SPIE Digital Library.
PERSONAL Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.