One limitation of microendoscopy is the device footprint that should be minimal for many applications. Here we present a minimally-invasive endoscope based on a multimode fiber that combines photoacoustic and fluorescence sensing. With the use of a fast spatial-light modulator, it is possible to rapidly learn the transmission matrix during a prior calibration step. A focused spot can then be produced to raster-scan a sample at the distal tip of the fiber. Our setup provides both photoacoustic and fluorescence microscopic images of test samples in vitro (fluorescent beads and red blood cells) through a single multimode fiber.
Many applications of microendoscopy, including brain imaging, requires minimally invasive devices to minimize damage during insertion in the tissue. Here we present a minimally-invasive endoscope based on a multimode fiber that combines photoacoustic and fluorescence sensing. By learning the transmission matrix during a prior calibration step, a focused spot can be produced and raster-scanned over a sample at the distal tip of the fiber by use of a spatial light modulator. We demonstrate that our setup provides both photoacoustic and fluorescence microscopic images of test samples in vitro (fluorescent beads and red blood cells) through the same fiber.
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