Dr. Rudolf Oldenbourg Profile

Dr. Rudolf Oldenbourg

Senior Scientist at Marine Biological Lab.

SPIE Involvement:

Author

Proceedings Article | 10 September 2004 Paper

Mapping polymer birefringence in three-dimensions using a polarizing microscope with oblique illumination

Michael Shribak, Rudolf Oldenbourg

Proceedings Volume 5462, (2004) https://doi.org/10.1117/12.549050

KEYWORDS: Birefringence, Microscopes, Polarization, Wave plates, Ocean optics, Liquid crystals, Linear polarizers, Polarizers, Refractive index, Crystal optics

Read Abstract +

We have been developing a polarized light microscope with liquid crystal universal compensator and circular polarizer (the LC-PolScope) for recording images, which are independent of the orientation of birefringent objects. Separate images show the retardance and the slow axis azimuth distributions of the in-plane birefringence of the focused region in the specimen. However the measured (apparent) retardance still depends on the angle between the crystal optic axis and the axis of the illuminating beam of light. If the illuminating beam is close to parallel to the optic axis the measured retardance value decreases dramatically and becomes zero when the two axes are parallel. The description of birefringent objects oriented in 3-dimensional space requires the introduction of two additional parameters: the principal retardance and the inclination angle. Together with the azimuth angle they completely characterize the birefringence properties of a specimen, assuming the specimen has a uniaxial optical indicatrix. We devised a new technique for measuring the three birefringence parameters without moving the specimen. For exploring the out-of-plane birefringence the new instrument which is based on the LC-PolScope technique contains an additional spatial light modulator, implemented here as a liquid crystal mask. The mask is located in the aperture plane of the condenser lens. Partial occlusion of the condenser aperture changes the direction of the central ray of the cone of light converging on the specimen. So we can obtain the retardance and azimuth images using different sets of illumination rays. For experimental verification we used a biological object called an aster. An aster consists of nearly parallel arrays of microtubules, a stiff biopolymer, radiating from a common organizing center called a centrosome. The object is spherically symmetric, and its 3 dimensional distribution of birefringence orientation can be predicted. Experimental results have shown the developed polarizing microscope can successfully be used for imaging and measuring three-dimensional orientation of birefringent objects

Proceedings Article | 25 September 2002 Paper

Sensitive measurements of two-dimensional birefringence distributions using near-circularly polarized beam

Michael Shribak, Rudolf Oldenbourg

Proceedings Volume 4819, (2002) https://doi.org/10.1117/12.463750

KEYWORDS: Wave plates, Polarization, Birefringence, Liquid crystals, Polarizers, Microscopes, Imaging systems, Time metrology, Linear polarizers, Sensors

Read Abstract +

Proceedings Article | 15 May 2002 Paper

Scanning aperture polarized light microscope: observation of small calcite crystals using oblique illumination

Michael Shribak, Rudolf Oldenbourg

Proceedings Volume 4621, (2002) https://doi.org/10.1117/12.467840

KEYWORDS: Crystals, Microscopes, Calcite, Birefringence, Crystal optics, Liquid crystals, Polarization, Wave plates, Refractive index, Objectives

Read Abstract +

SPIE Journal Paper | 1 May 2002

Polarization aberrations caused by differential transmission and phase shift in high numerical aperture lenses: theory, measurement, and retification

Michael Shribak, Shinya Inoue, Rudolf Oldenbourg

OE, Vol. 41, Issue 05, (May 2002) https://doi.org/10.1117/12.10.1117/1.1467669

KEYWORDS: Polarization, Lenses, Microscopes, Objectives, Refractive index, Phase shifts, Polarizers, Glasses, Optical testing, Mineralogy

Proceedings Article | 9 January 2002 Paper

Rectifiers for suppressing depolarization caused by differential transmission and phase shift in high NA lenses

Michael Shribak, Shinya Inoue, Rudolf Oldenbourg

Proceedings Volume 4481, (2002) https://doi.org/10.1117/12.452885

KEYWORDS: Polarization, Lenses, Microscopes, Refractive index, Birefringence, Glasses, Polarizers, Objectives, Phase shifts, Liquid crystals

Read Abstract +

Proceedings Article | 2 November 2001 Paper

Dynamic views of living cell fine structure revealed by birefringence imaging

Rudolf Oldenbourg

Proceedings Volume 4431, (2001) https://doi.org/10.1117/12.447445

KEYWORDS: Birefringence, Microscopes, Spindles, Ocean optics, Neurons, Optical fibers, Organisms, Fluorescent markers, Optical imaging, Optical properties

Read Abstract +

Proceedings Article | 16 April 2001 Paper

3D imaging properties of a polarized light microscope revealed by birefringence measurements of small calcite crystals

Michael Shribak, Rudolf Oldenbourg

Proceedings Volume 4261, (2001) https://doi.org/10.1117/12.424522

KEYWORDS: Crystals, Calcite, Microscopes, Wave plates, Birefringence, Liquid crystals, Crystal optics, Superposition, Objectives, Polarization

Read Abstract +

Proceedings Article | 14 September 1994 Paper

Fast imaging polarimetry with precision universal compensator

Guang Mei, Rudolf Oldenbourg

Proceedings Volume 2265, (1994) https://doi.org/10.1117/12.186681

KEYWORDS: Wave plates, Microscopes, Cameras, Polarimetry, Polarization, Video, Liquid crystals, Optical testing, Anisotropy, Linear polarizers

Read Abstract +

Showing 5 of 8 publications

Conference Committee Involvement (7)

Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XVI

27 January 2009 | San Jose, California, United States

Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XV

21 January 2008 | San Jose, California, United States

Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XIV

24 January 2007 | San Jose, California, United States

Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XIII

24 January 2006 | San Jose, California, United States

Three-Dimensional and Multidimensional Microscopy: Image Processing and Acquisition XII

26 January 2005 | San Jose, CA, United States

Showing 5 of 7 Conference Committees

Keywords/Phrases

Search In:

Publication Years