Silicon photonic devices, such as SOI microring resonators, have optical properties that are incredibly responsive to changes in the local dielectric environment accompanying a biological binding event at or near the ring surface. We have developed a platform in which arrays of uniquely addressable microrings are functionalized with target-specific capture agents so as to create a powerful tool for multiplexed biomolecular detection. We will discuss applications of this technology to the multiplexed detection of DNA, RNA, and proteins in clinically-relevant matrices, and will also describe the rigorous empirical determination of key sensitivity metrics.
Silicon photonic technology has incredible potential to transform multiplexed bioanalysis on account of the scalability of
device fabrication, which maps favorably to a myriad of medical diagnostic applications. The optical properties of
CMOS-fabricated microring resonators are incredibly responsive to changes in the local dielectric environment
accompanying a biological binding event near the ring surface. Arrays of high-Q microrings were designed to be
individually addressable both in surface derivitization, using well-established microarraying technologies, and in optical
evaluation. The optical response of each ring can be determined in near real time allowing multiple biomolecular
interactions to be simultaneously monitored. We describe a stable and robust measurement platform that allows sensitive
visualization of small molecule surface chemical derivitization as well as monitoring of biological interactions, including
the detection of proteins and nucleic acids. We also present recent results demonstrating multiplexed measurement of
cancer markers. These demonstrations establish a pathway to higher level multiparameter analysis from real-world
patient samples; a development that will enable individualized disease diagnostics and personalized medicine.
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