LightPath Technologies scales up chalcogenide glass for optics production. Understanding fabrication effects on refractive index and ensuring repeatability are crucial. They employ TRIOPTICS OptiSurf LTM system to investigate refractive variability. This system uses low-coherence interferometry to measure lens thickness and air gaps. Direct measurement requires group index knowledge for geometric thickness determination. TRIOPTICS GmbH addresses this with LensGage, an OptiSurf addon. LensGage consists of calibrated parallel plates, accurately determining geometric thickness and group index (+/-0.75nm and 0.0001 precision) in a single measurement at 1.31µm or 2.2µm. LensGage enables LightPath to improve quality and consistency of fabricated optics by enhancing their understanding of group index variability.
Understanding the structure and function of vasculature in the brain is important for both the interpretation of medical functional imaging data and the mechanistic understanding of neurovascular disorders. Currently, there lacks a volumetric vasculature imaging method able to uncover distributed hemodynamics at sub-capillary resolution and blood flow-resolving speed. Here, we applied two-photon microscopy with an axially extended Bessel focus to fast volumetric imaging of vasculature in the awake brain. We measured vessel dilation, constriction, and 3-dimensional blood flow at unprecedentedly high throughput and capillary resolution. We expect our method to make broad impacts on studying neurovasculature in vivo.
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