Dr. Tali Ilovitsh Profile

Dr. Tali Ilovitsh

Post doctoral reasercher at Bar Ilan University

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Author

Publications (8)

Proceedings Article | 24 April 2017 Presentation

Phase retrieval and 3D imaging in gold nanoparticles based fluorescence microscopy (Conference Presentation)

Tali Ilovitsh, Asaf Ilovitsh, Aryeh Weiss, Rinat Meir, Zeev Zalevsky

Proceedings Volume 10074, 1007406 (2017) https://doi.org/10.1117/12.2251587

KEYWORDS: Luminescence, 3D image processing, 3D acquisition, Image processing, 3D image enhancement, Phase retrieval, Stereoscopy, Microscopy, Gold, Nanoparticles

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Proceedings Article | 22 February 2017 Presentation + Paper

Time multiplexing super-resolution nanoscopy based on the Brownian motion of gold nanoparticles

Tali Ilovitsh, Asaf Ilovitsh, Omer Wagner, Zeev Zalevsky

Proceedings Volume 10077, 100770R (2017) https://doi.org/10.1117/12.2250277

KEYWORDS: Nanoparticles, Super resolution, Gold, Microscopy, Image processing, Particles, Signal to noise ratio, Point spread functions, Lawrencium, Microscopes

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SPIE Journal Paper | 8 September 2016

Silicon-coated gold nanoparticles nanoscopy

Yossef Danan, Tali Ilovitsh, Yehonatan Ramon, Dror Malka, Danping Liu, Zeev Zalevsky

JNP, Vol. 10, Issue 03, 036015, (September 2016) https://doi.org/10.1117/12.10.1117/1.JNP.10.036015

KEYWORDS: Silicon, Point spread functions, Nanoparticles, Gold, Refractive index, Nanostructures, Super resolution, Super resolution microscopy, Coating, Laser beam diagnostics

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Proceedings Article | 22 April 2016 Presentation + Paper

Plasma dispersion effect assisted nanoscopy based on tuning of absorption and scattering resonances of nanoparticles

Yossef Danan, Tali Ilovitsh, Danping Liu, Hadar Pinhas, Moshe Sinvani, Yehonatan Ramon, Jonathan Azougi, Alexandre Douplik, Zeev Zalevsky

Proceedings Volume 9721, 97210J (2016) https://doi.org/10.1117/12.2210800

KEYWORDS: Nanoparticles, Absorption, Scattering, Silicon, Plasma, Light scattering, Super resolution microscopy, Medical diagnostics, Coating, Gold, Point spread functions, Refractive index

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Proceedings Article | 22 April 2016 Presentation + Paper

Temporally flickering nanoparticles for compound cellular imaging and super resolution

Tali Ilovitsh, Yossef Danan, Rinat Meir, Amihai Meiri, Zeev Zalevsky

Proceedings Volume 9721, 97210V (2016) https://doi.org/10.1117/12.2208864

KEYWORDS: Nanoparticles, Super resolution, Gold, Modulation, Signal to noise ratio, Light scattering, Image processing, Microscopy, Inspection, Biomedical optics, Interference (communication), Image enhancement, Satellite navigation systems, Ions

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Proceedings Article | 9 March 2016 Presentation + Paper

Time multiplexing super resolution using a 2D Barker-based array

Asaf Ilovitsh, Tali Ilovitsh, Eyal Preter, Nadav Levanon, Zeev Zalevsky

Proceedings Volume 9716, 97160I (2016) https://doi.org/10.1117/12.2206198

KEYWORDS: Super resolution, Multiplexing, Computer programming, Image resolution, Image processing, Phase only filters, Optical imaging, Image analysis, Lawrencium, Spatial light modulators, Cameras, Point spread functions, Numerical simulations

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Proceedings Article | 9 March 2016 Presentation + Paper

Modified K-factor image decomposition for three-dimensional super resolution microscopy

Tali Ilovitsh, Aryeh Weiss, Amihai Meiri, Carl Ebeling, Aliza Amiel, Hila Katz, Batya Mannasse-Green, Zeev Zalevsky

Proceedings Volume 9713, 97131R (2016) https://doi.org/10.1117/12.2205970

KEYWORDS: 3D image processing, Point spread functions, 3D modeling, 3D image enhancement, Microscopy, Image processing, Data modeling, Microscopes, Super resolution microscopy, Luminescence, 3D acquisition, Computer simulations, Biomedical optics

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The ability to track single fluorescent particles within a three dimensional (3D) cellular environment can provide valuable insights into cellular processes. In this paper, we present a modified nonlinear image decomposition technique called K-factor that reshapes the 3D point spread function (PSF) of an XYZ image stack into a narrow Gaussian profile. The method increases localization accuracy by ~60% with compare to regular Gaussian fitting, and improves minimal resolvable distance between overlapping PSFs by ~50%. The algorithm was tested both on simulated data and experimentally.

This work presets a novel use of the nonlinear image decomposition technique called K-factor that reshapes the three dimensional (3D) point spread function (PSF) of an XYZ image stack into a narrow Gaussian profile. The experimentally obtained PSF of a Z-stack raw data that is acquired by a widefield microscope has a more elaborate shape that is given by the Gibson and Lanni model. This shape increases the computational complexity associated with the localization routine, when used in localization microscopy techniques. Furthermore, due to its nature, this PSF spreads over a larger volume, making the problem of overlapping emitters detection more pronounced. The ability to use Gaussian fitting with high accuracy on 3D data can facilitate the computational complexity, hence reduce the processing time required for the generation of the 3D superresolved image. In addition it allows the detection of overlapping PSFs and reduces the effects of the penetration of out of focus PSFs into in focused PSFs, therefore enables the increase in the activated fluorophore density by ~50%. The algorithm was tested both on simulated data and experimentally, where it yielded an increase in the localization accuracy by ~60% with compare to regular Gaussian fitting, and improved the minimal resolvable distance between overlapping PSFs by ~50%, making it extremely applicable to the field of 3D biomedical imaging,

Proceedings Article | 9 March 2016 Presentation + Paper

Three-dimensional imaging using phase retrieval with two focus planes

Tali Ilovitsh, Asaf Ilovitsh, Aryeh Weiss, Rinat Meir, Zeev Zalevsky

Proceedings Volume 9713, 97130Y (2016) https://doi.org/10.1117/12.2205769

KEYWORDS: 3D image processing, Phase retrieval, Microscopy, Stereoscopy, Laser scattering, Absorption, Gold, Nanoparticles, Free space optics, Optical microscopy, Reconstruction algorithms, Image processing, 3D image reconstruction, 3D acquisition, Point spread functions, Evolutionary algorithms

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Showing 5 of 8 publications

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