Mr. Yong-Jin Cho Profile

Yong-Jin Cho

at Yonsei Univ

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Proceedings Article | 2 March 2010 Paper

5-ALA induced fluorescent image analysis of actinic keratosis

Yong-Jin Cho, Youngwoo Bae, Eung-Ho Choi, Byungjo Jung

Proceedings Volume 7548, 75480J (2010) https://doi.org/10.1117/12.843050

KEYWORDS: Luminescence, Hyperspectral imaging, Image analysis, Signal detection, Image segmentation, Photodynamic therapy, Tissues, Color imaging, Skin cancer, Principal component analysis

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In this study, we quantitatively analyzed 5-ALA induced fluorescent images of actinic keratosis using digital fluorescent color and hyperspectral imaging modalities. UV-A was utilized to induce fluorescent images and actinic keratosis (AK) lesions were demarcated from surrounding the normal region with different methods. Eight subjects with AK lesion were participated in this study. In the hyperspectral imaging modality, spectral analysis method was utilized for hyperspectral cube image and AK lesions were demarcated from the normal region. Before image acquisition, we designated biopsy position for histopathology of AK lesion and surrounding normal region. Erythema index (E.I.) values on both regions were calculated from the spectral cube data. Image analysis of subjects resulted in two different groups: the first group with the higher fluorescence signal and E.I. on AK lesion than the normal region; the second group with lower fluorescence signal and without big difference in E.I. between two regions. In fluorescent color image analysis of facial AK, E.I. images were calculated on both normal and AK lesions and compared with the results of hyperspectral imaging modality. The results might indicate that the different intensity of fluorescence and E.I. among the subjects with AK might be interpreted as different phases of morphological and metabolic changes of AK lesions.

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