A new digital technique for objective differential diagnosis of the septic process severity was developed and experimentally tested by phase mapping of microscopic images of histological sections of polycrystalline internal organs and blood films of laboratory rats. the results of statistical analysis of histological sections of the internal organs of rats from control group 1 and research groups 2 to 4 with different severity of septic pathology are presented.
The work is aimed at the development and experimental testing of the polarization-correlation method for the analysis of the polycrystalline structure of biological layers. introduced a new parameter-complex degree of mutual anisotropy. the statistical (statistical moments of the 1st – 4th orders) structure of the distributions of the values of the cdma module of spatially structured fibrillar networks of histological sections of the myocardium that died due to mechanical asphyxia and heart attack was experimentally studied.
The work is aimed at the development and experimental testing of the polarization-spectral method for analyzing the polycrystalline structure of the synovial fluid films of a human joint. comparing the sensitivity, specificity and accuracy of the methods of polarimetry in the diagnosis and differentiation of the above pathological conditions of the joints, we can conclude: the highest sensitivity, specificity and accuracy are demonstrated by the method of spectropolarimetry, sensitivity reaches 92 - 93%, specificity - 82 - 86%, and accuracy - 88% - 89%. additionally, the possibility of diagnosing the type of brain stroke by changing the polarization spectral characteristics is considered.
The results of applying algorithms for reconstructing parameters characterizing the linear dichroism of networks of biological crystals in differentiating changes in optical anisotropy associated with varying degrees of severity of pathology, pre-cancerous (atrophy and endometrial polyp) states of the cervix are presented.
This report contains theoretical algorithm for the differential representation of a phaseinhomogeneous biological object as a set of consecutively located optically anisotropic layers; theoretical algorithms for the decomposition of the Mueller-matrix of the diffuse biological layer in the basis of differential matrices of the 1st and 2nd orders; analytical relations for determining the magnitude of the set of elements of differential matrices of the 1st and 2nd orders;information and characteristics of optical schemes of experimental devices.
The results of a statistical analysis of the distributions of characteristic values of the Stokes vector fourth parameter (crystallization parameter - CP) of the digital vector-parametric microscopic image of healthy spleen and with spleen with sepsis histological sections are presented. We determined the statistical significance of differentiation of vector-parametric maps of the microscopic image of histological sections of rat spleens from control group 1 and research groups 2-4 with different severity of septic pathology. The most diagnostic-sensitive statistical criteria for differentiating vector-parametric maps of microscopic images of histological sections of the spleen are established.
A new digital technique of objective differential diagnosis of the septic process severity was developed and tested experimentally by polarization-phase mapping of microscopic images of histological sections of the liver of laboratory rats based on statistical and information analysis of phase shift distributions.
For differential diagnosis of severity of the septic process, the method of mapping vector-parametric microscopic images of the polycrystalline component of histological sections of the internal organs of rats was used. A statistical analysis of the distribution of the number of characteristic values of the Stokes vector of polarizing microscopic images of histological sections of the internal organs of laboratory rats was carried out. The relationships between the values of statistical moments of the 1st - 2nd orders of magnitude characterizing the distribution of the number of characteristic values of the Stokes vector of polarizing microscopic images of histological sections of the internal organs of laboratory rats and the severity of the septic process are determined. Quantitative parameters that are most sensitive to septic conditions have been established, which ensure the implementation of statistically confidance differentiation of histological sections of the internal organs of laboratory rats. From the standpoint of evidence-based medicine, the operational characteristics of the diagnostic forces of the vector-parametric microscopy method are established with an estimate of the number of characteristic values of the fourth Stokes vector of polarization images of histological sections of the internal organs of laboratory rats.
In this paper are presented: structural-logical diagram and design of a phasemetric study of microscopic images of blood films of laboratory rats; model analysis of the polycrystalline structure of blood films of laboratory rats; structurally logical diagram and characteristics of the optical arrangement of the system of phasemetric mapping of microscopic images of blood films of laboratory rats; algorithms for statistical processing of experimental data of a system for phasemetric mapping of microscopic images of blood films of laboratory rats; information analysis algorithms to determine the strength of the diagnostic method of phasemetric mapping of microscopic images of blood films of laboratory rats by establishing a set of operational characteristics - sensitivity, specificity and accuracy.
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