Cellular resolution of optical coherence tomography (OCT) is vital to achieve precise diagnosis by offering high-quality images of virtual biopsy. Currently, the common solution is to apply dynamic focusing to axially translate the focus through the region of interest with a high numerical aperture (N.A.) objective, followed by Z-stacking to rebuild a high-resolution 3D volume. To accelerate the imaging acquisition, this work developed metasurface optical plates to generate multiple foci along axial direction. Two-/three-/seven-foci had been testified with bead phantom using a scanning OCT. Human skin and human brain samples were imaged with cellular resolution.
In this work, we developed a new method for high throughput imaging flow cytometry, using diffractive optics elements to generate linear laser spot array for illumination, and single-pixel detectors for detection. The illumination spots are arranged in a line at equal intervals and form a small angle with the direction of the cell movement. When the cell passes through the illumination area, the two-dimensional information of the cell's fluorescence and scattered intensity profile is encoded into signals detected by the PMTs. Fluorescence and scattering imaging were experimentally demonstrated for beads and cells traveling at a velocity of 4.7 m/s in a microfluidic chip, with a resolution of 1 μm and a maximum throughput of 5000cell/s.
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