Photothermal Refraction For Scanning Laser Microscopy

Dean S. Burgi; Thomas G. Nolan; Jon A. Rusfelt; Norman J. Dovichi

doi:10.1117/12.7973377

1 December 1984 Photothermal Refraction For Scanning Laser Microscopy

Dean S. Burgi, Thomas G. Nolan, Jon A. Rusfelt, Norman J. Dovichi

Author Affiliations +

Optical Engineering, Vol. 23, Issue 6, 236756 (December 1984). https://doi.org/10.1117/12.7973377

Abstract

Photothermal refraction is a novel thermo-optical technique that measures absorbance from localized regions within larger samples. In this technique, a modulated pump laser beam and a coplanar continuous-wave probe laser beam cross at right angles within the sample. Absorbance of the pump laser beam generates a time-varying localized temperature rise within the sample. This heated region acts as a thermal cylindrical lens that periodically defocuses the probe beam and results in a change in the probe beam center intensity. Since the pump and probe beams interact only in their intersection volume, a localized measure of sample absorbance is produced. A high spatial resolution image of sample absorbance may be made by recording the photothermal refraction signal as the sample is scanned through the intersection of the laser beams.

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Dean S. Burgi, Thomas G. Nolan, Jon A. Rusfelt, and Norman J. Dovichi "Photothermal Refraction For Scanning Laser Microscopy," Optical Engineering 23(6), 236756 (1 December 1984). https://doi.org/10.1117/12.7973377

Published: 1 December 1984

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