Materials, Photonic Devices, and Sensors

Stability optimization of microbial surface-enhanced Raman spectroscopy detection with immunomagnetic separation beads

[+] Author Affiliations
Sanna Uusitalo, Jarno Petäjä, Jussi Hiltunen

VTT Technical Research Centre of Finland, Oulu, Finland

Martin Kögler

University of Helsinki, Centre for Drug Research, Division of Pharmaceutical Biosciences, Faculty of Pharmacy, Helsinki, Finland

Technische Universität Berlin, Chair of Bioprocess Engineering, Institute of Biotechnology, Berlin, Germany

Anna-Liisa Välimaa, Riitta Laitinen

National Resources Institute Finland (LUKE), Bio-based Business and Industry, Oulu, Finland

Ville Kontturi, Samuli Siitonen

Nanocomp Oy Ltd., Lehmo, Finland

Matti Kinnunen

University of Oulu, Faculty of Information Technology and Electrical Engineering, Optoelectronics and Measurement Techniques Research Unit, Oulu, Finland

Tapani Viitala

University of Helsinki, Centre for Drug Research, Division of Pharmaceutical Biosciences, Faculty of Pharmacy, Helsinki, Finland

Opt. Eng. 56(3), 037102 (Mar 07, 2017). doi:10.1117/1.OE.56.3.037102
History: Received December 22, 2016; Accepted February 17, 2017
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Abstract.  Immunomagnetic separation (IMS) beads with antibody coating are an interesting option for biosensing applications for the identification of biomolecules and biological cells, such as bacteria. The paramagnetic properties of the beads can be utilized with optical sensing by migrating and accumulating the beads and the bound analytes toward the focus depth of the detection system by an external magnetic field. The stability of microbial detection with IMS beads was studied by combining a flexible, inexpensive, and mass producible surface-enhanced Raman spectroscopy (SERS) platform with gold nanoparticle detection and antibody recognition by the IMS beads. Listeria innocua ATCC 33090 was used as a model sample and the effect of the IMS beads on the detected Raman signal was studied. The IMS beads were deposited into a hydrophobic sample well and accumulated toward the detection plane by a neodymium magnet. For the first time, it was shown that the spatial stability of the detection could be improved up to 35% by using IMS bead capture and sample well placing. The effect of a neodymium magnet under the SERS chip improved the temporal detection and significantly reduced the necessary time for sample stabilization for advanced laboratory testing.

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© 2017 Society of Photo-Optical Instrumentation Engineers

Citation

Sanna Uusitalo ; Martin Kögler ; Anna-Liisa Välimaa ; Jarno Petäjä ; Ville Kontturi, et al.
"Stability optimization of microbial surface-enhanced Raman spectroscopy detection with immunomagnetic separation beads", Opt. Eng. 56(3), 037102 (Mar 07, 2017). ; http://dx.doi.org/10.1117/1.OE.56.3.037102


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