Dynamic light-scattering studies of BSA and lysozyme using a backscatter fiber optic system

Harbans S. Dhadwal; William W. Wilson; Rafat R. Ansari; William V. Meyer

doi:10.1117/12.148349

21 July 1993 Dynamic light-scattering studies of BSA and lysozyme using a backscatter fiber optic system

Harbans S. Dhadwal, William W. Wilson, Rafat R. Ansari, William V. Meyer

Proceedings Volume 1884, Static and Dynamic Light Scattering in Medicine and Biology; (1993) https://doi.org/10.1117/12.148349
Event: OE/LASE'93: Optics, Electro-Optics, and Laser Applications in Scienceand Engineering, 1993, Los Angeles, CA, United States

Abstract

A comparative dynamic light scattering study of BSA and lysozyme is presented. A backscatter fiber optic system and a conventional light scattering spectrometer are used to measure the diameter of proteins in the dilute regime, that is, below concentrations of 10 mg/ml. The fiber optic system operating with a power level of 2 mW at a wavelength of 632.8 nm compares favorably with a conventional system operating with a power level of 40 mW at a wavelength of 514.5 nm. Quasi-elastic light scattering measurements taken at several concentrations demonstrate the utility of a backscatter fiber optic probe for sizing of small molecular weight proteins. The fiber probe, comprising two optical fibers, is about 3 mm in diameter and can be positioned either inside or outside the scattering cell.

Citation Download Citation

Harbans S. Dhadwal, William W. Wilson, Rafat R. Ansari, and William V. Meyer "Dynamic light-scattering studies of BSA and lysozyme using a backscatter fiber optic system", Proc. SPIE 1884, Static and Dynamic Light Scattering in Medicine and Biology, (21 July 1993); https://doi.org/10.1117/12.148349

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