Video imaging in flow - a novel method for increasing the discriminating power of flow cytometers used in aquatic science

Joachim Wietzorrek; E. Glossner; R. Kuehn; V. Kachel

doi:10.1117/12.168746

15 February 1994 Video imaging in flow - a novel method for increasing the discriminating power of flow cytometers used in aquatic science

Joachim Wietzorrek, E. Glossner, R. Kuehn, V. Kachel

Author Affiliations +

Proceedings Volume 2085, Biochemical and Medical Sensors; (1994) https://doi.org/10.1117/12.168746
Event: Europto Biomedical Optics '93, 1993, Budapest, Hungary

Abstract

The procedure of imaging in flow, evoked by the need for simplified and reliable particle identification in aquatic science, means taking cell images directly from the transducer of a flow cytometer. From that point of view imaging in flow is the link of the traditional microscopical method and the modern flow cytometric technique for analyzing particles. Principal problems rising from that approach are discussed in this paper. This encloses positioning of the objects to be imaged, design criteria for illumination and imaging optics, the procedure for taking asynchronous video images and requirements concerning the light source.

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Joachim Wietzorrek, E. Glossner, R. Kuehn, and V. Kachel "Video imaging in flow - a novel method for increasing the discriminating power of flow cytometers used in aquatic science", Proc. SPIE 2085, Biochemical and Medical Sensors, (15 February 1994); https://doi.org/10.1117/12.168746

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