Here, we present vFLETCHERS (visible fluorescence-encoded time-domain coherent Raman spectroscopy), which operates in the visible excitation region and overcomes the previous limitation of detectable fluorophores. vFLETCHERS employs a non-collinear optical parametric amplifier as a femtosecond excitation source. As a proof-of-concept demonstration, we acquired low-frequency Raman spectra (<1000 cm-1) of solutions containing commercial fluorophores with the absorption peaks in the 600-700 nm region. These results highlight the potential of vFLETCHERS as a versatile multiplexed imaging technique, opening up new opportunities for research in biology.
Access to the requested content is limited to institutions that have purchased or subscribe to SPIE eBooks.
You are receiving this notice because your organization may not have SPIE eBooks access.*
*Shibboleth/Open Athens users─please
sign in
to access your institution's subscriptions.
To obtain this item, you may purchase the complete book in print or electronic format on
SPIE.org.
INSTITUTIONAL Select your institution to access the SPIE Digital Library.
PERSONAL Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.