Laser Induced photolysis reveals a role of ATP in traumatic brain injury repair

Nicole Wakida; Vincent Nguyen; Michael W. Berns

doi:10.1117/12.2633131

13 October 2022 Laser Induced photolysis reveals a role of ATP in traumatic brain injury repair

Nicole Wakida, Vincent Nguyen, Michael W. Berns

Proceedings Volume PC12198, Optical Trapping and Optical Micromanipulation XIX; PC1219804 (2022) https://doi.org/10.1117/12.2633131
Event: SPIE Nanoscience + Engineering, 2022, San Diego, California, United States

Abstract

The use of laser nanosurgery to induce photolysis has proven to be an indispensable tool for in vitro studies of astrocytes. Here, we utilize laser nanosurgery to initiate damage within single astrocytes in an vitro traumatic brain injury model. Changes in cytoplasmic ATP levels were observed throughout the astrocyte network following the targeted lysis of a single cell. In response to the death of a neighboring cell, a transient drop in cytoplasmic ATP levels was observed. This combined method of optical technologies should prove valuable in understanding astrocytes’ role in detection of nervous tissue damage.

Conference Presentation

Citation Download Citation

Nicole Wakida, Vincent Nguyen, and Michael W. Berns "Laser Induced photolysis reveals a role of ATP in traumatic brain injury repair", Proc. SPIE PC12198, Optical Trapping and Optical Micromanipulation XIX, PC1219804 (13 October 2022); https://doi.org/10.1117/12.2633131

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