The use of laser nanosurgery to induce photolysis has proven to be an indispensable tool for in vitro studies of astrocytes. Here, we utilize laser nanosurgery to initiate damage within single astrocytes in an vitro traumatic brain injury model. Changes in cytoplasmic ATP levels were observed throughout the astrocyte network following the targeted lysis of a single cell. In response to the death of a neighboring cell, a transient drop in cytoplasmic ATP levels was observed. This combined method of optical technologies should prove valuable in understanding astrocytes’ role in detection of nervous tissue damage.
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