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Scanning microscopy’s resolution of highly localized dynamics in awake animals is limited by bulk motion. We present a modular optical flow sensor based on spectral domain OCT which measures the specimen’s 3D displacement in real-time. A rose-function scan pattern rapidly acquires B-lines used to estimate displacements via an adaptive cross-correlation approach. Axial displacements are estimated from phase fluctuations apparent in the cross-correlation of the complex SD-OCT signal. The technique’s 3-dimensional readout rate and sensitivity to physiologically-relevant motion frequencies and magnitudes is evaluated.
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Stephen Tucker, Michael Wallace, Jianbo Tang, Anderson Chen, David Boas, "Real-time optical flow sensing with SD-OCT: towards a modular motion-correction platform for microscopy," Proc. SPIE 11649, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXVIII, 1164917 (5 March 2021); https://doi.org/10.1117/12.2578380