Alejandro De la Cadena,1 Andrea Ragni,1 Giuseppe Sciortino,1 Federico Vernuccio,1 Carlo Valensise,1 Marco Sampietro,1 Giorgio Ferrari,1 Giulio Cerullo,1 Dario Pollihttps://orcid.org/0000-0002-6960-57081
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Stimulated Raman scattering (SRS) microscopy is a powerful technique for label-free identification of molecules based on their intrinsic vibrational spectrum. We present a novel approach to broadband SRS microscopy based on a recently developed balanced low-noise integrated-circuit multichannel lock-in amplifier with 10-μs integration time and 32 channels working in parallel, enabling multiplex SRS detection at speed faster than one hyperspectral frame per second. The system is powered by a narrowband Stokes pulse at 1040 nm and a broadband (approx. 500 cm^-1) pump pulse generated by a home-built low-noise optical parametric oscillator with up to 100-mW average power, covering the whole C-H stretching band. We measure stimulated Raman loss on the broadband pump pulse employing an in-line balanced detection approach to suppress the laser fluctuations and achieve close to shot-noise-limited sensitivity. We will show the system performances in high-resolution cell imaging.
Alejandro De la Cadena,Andrea Ragni,Giuseppe Sciortino,Federico Vernuccio,Carlo Valensise,Marco Sampietro,Giorgio Ferrari,Giulio Cerullo, andDario Polli
"Broadband stimulated Raman scattering microscopy at >1 frame/s with a multi-channel integrated-circuit differential lock-in amplifier", Proc. SPIE 11656, Advanced Chemical Microscopy for Life Science and Translational Medicine 2021, 116560V (5 March 2021); https://doi.org/10.1117/12.2577680
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Alejandro De la Cadena, Andrea Ragni, Giuseppe Sciortino, Federico Vernuccio, Carlo Valensise, Marco Sampietro, Giorgio Ferrari, Giulio Cerullo, Dario Polli, "Broadband stimulated Raman scattering microscopy at >1 frame/s with a multi-channel integrated-circuit differential lock-in amplifier," Proc. SPIE 11656, Advanced Chemical Microscopy for Life Science and Translational Medicine 2021, 116560V (5 March 2021); https://doi.org/10.1117/12.2577680