Laser induced shockwave paired with FRET to study neuronal responses to shear stress

Veronica Gomez Godinez; Vikash Morar; Christopher Carmona; Yingli Gu; Kijung Sung; Linda Z. Shi; Chengbiao Wu; Daryl Preece; Michael W. Berns

doi:10.1117/12.2595803

3 August 2021 Laser induced shockwave paired with FRET to study neuronal responses to shear stress

Veronica Gomez Godinez, Vikash Morar, Christopher Carmona, Yingli Gu, Kijung Sung, Linda Z. Shi, Chengbiao Wu, Daryl Preece, Michael W. Berns

Author Affiliations +

Proceedings Volume 11798, Optical Trapping and Optical Micromanipulation XVIII; 117982H (2021) https://doi.org/10.1117/12.2595803
Event: SPIE Nanoscience + Engineering, 2021, San Diego, California, United States

Abstract

Laser induced shockwave (LIS) can be utilized to subject neuronal cells to conditions similar to those occurring during a blast induced traumatic brain injury. We utilized a 532nm Coherent Flare laser to induce a shockwave near cells which had been transfected with a FRET calcium biosensor (D3CPV) so that we could monitor the immediate cellular responses. Our shockwave system was characterized with a high-speed camera to monitor cavitation bubble dynamics and calculate the shear forces cells were subjected to. We found that we could induce forces which have been previously shown to induce injury. Using both phase and fluorescence microscopy we monitored the effects of shear on our cells. We found that at distances up to 120 microns from the laser focal point cells experienced shears greater than 10kPa. At those distances cell fragmentation was observed. Cells that survived and expressed the FRET biosensor demonstrated an immediate calcium elevation irrespective of extracellular or cytoplasmic calcium concentration. Cells recovered to pre-shockwave calcium levels within ~30s. In conclusion, LIS can be utilized to simultaneously monitor the neuronal response to shear stress and nearby cell death or injury.

Citation Download Citation

Veronica Gomez Godinez, Vikash Morar, Christopher Carmona, Yingli Gu, Kijung Sung, Linda Z. Shi, Chengbiao Wu, Daryl Preece, and Michael W. Berns "Laser induced shockwave paired with FRET to study neuronal responses to shear stress", Proc. SPIE 11798, Optical Trapping and Optical Micromanipulation XVIII, 117982H (3 August 2021); https://doi.org/10.1117/12.2595803

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