Conformation changes in bacteriorhodopsin photocycle studied by pump and probe tryptophan fluorescence decay

G. I Groma; Gabor Szabo; L. Keszthelyi

doi:10.1117/12.146116

17 June 1993 Conformation changes in bacteriorhodopsin photocycle studied by pump and probe tryptophan fluorescence decay

G. I Groma, Gabor Szabo, L. Keszthelyi

Author Affiliations +

Proceedings Volume 1921, Laser Spectroscopy of Biomolecules; (1993) https://doi.org/10.1117/12.146116
Event: Laser Spectroscopy of Biomolecules: 4th International Conference on Laser Applications in Life Sciences, 1992, Jyvaskyla, Finland

Abstract

Recent photoselection measurements on bacteriorhodospin at high pH and high ionic strength showed a high change of the anisotropy factor in the ms region. If this is due to a conformation change in the moiety of retinal chromophore, one can expect that it is also reflected in the fluorescence decay of tryptophan residues in the close vicinity of the chromophore. Time resolved tryptophan fluorescence measurements were carried out at different stages of bacteriorhodospin photocycle by a pump and probe method. Early results indicate a correlation between the time constant of the faster phase of the fluorescence decay and the anisotropy factor.

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G. I Groma, Gabor Szabo, and L. Keszthelyi "Conformation changes in bacteriorhodopsin photocycle studied by pump and probe tryptophan fluorescence decay", Proc. SPIE 1921, Laser Spectroscopy of Biomolecules, (17 June 1993); https://doi.org/10.1117/12.146116

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