Surface-enhanced FTIR spectroscopy and surface plasmon resonance on biomembranes

Gerald Steiner; Reiner Salzer; Wolfgang B. Fischer; Christian Kuhne

doi:10.1117/12.384946

8 May 2000 Surface-enhanced FTIR spectroscopy and surface plasmon resonance on biomembranes

Gerald Steiner, Reiner Salzer, Wolfgang B. Fischer, Christian Kuhne

Proceedings Volume 3918, Biomedical Spectroscopy: Vibrational Spectroscopy and Other Novel Techniques; (2000) https://doi.org/10.1117/12.384946
Event: BiOS 2000 The International Symposium on Biomedical Optics, 2000, San Jose, CA, United States

Abstract

Lipid bilayers containing the nicotinic acetylcholine receptor were investigated by attenuated total reflection (ATR) FTIR spectroscopy and by surface plasmon resonance (SPA). FTIR-ATR spectra reveal structural details of the transmembrane protein. Surface enhanced IR absorption provides access to structural details in the close vicinity of the surface of the ATR crystal. Information about the abundance of protein substructures as well as about the orientation of those substructures are obtained. SPR was applied in order to detect changes within the adsorbed biomembrane during admission of a neurotransmitter as acetylcholine. Switching of the ion channel was successfully by SPR. This result is a step forward towards highly selective biosensors and bioactuators.

Citation Download Citation

Gerald Steiner, Reiner Salzer, Wolfgang B. Fischer, and Christian Kuhne "Surface-enhanced FTIR spectroscopy and surface plasmon resonance on biomembranes", Proc. SPIE 3918, Biomedical Spectroscopy: Vibrational Spectroscopy and Other Novel Techniques, (8 May 2000); https://doi.org/10.1117/12.384946

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