Prof. Gerald Steiner Profile

Prof. Gerald Steiner

Research Associate at Universitaetsklinikum Carl Gustav Carus der TU Dresden

SPIE Involvement:

Author

Publications (38)

SPIE Journal Paper | 16 January 2025 Open Access

Comparative analysis of intraoperative thermal and optical imaging for identification of the human primary sensory cortex

Juliane Müller, Martin Oelschlägel, Stephan Sobottka, Matthias Kirsch, Gerald Steiner, Edmund Koch, Christian Schnabel

JBO, Vol. 30, Issue 01, 016002, (January 2025) https://doi.org/10.1117/12.10.1117/1.JBO.30.1.016002

KEYWORDS: Brain, Tumors, Optical imaging, Visualization, Surgery, Brain mapping, Biological research, Spatial resolution, Somatosensory cortex, Thermography

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SignificanceThe precise identification and preservation of functional brain areas during neurosurgery are crucial for optimizing surgical outcomes and minimizing postoperative deficits. Intraoperative imaging plays a vital role in this context, offering insights that guide surgeons in protecting critical cortical regions.AimWe aim to evaluate and compare the efficacy of intraoperative thermal imaging (ITI) and intraoperative optical imaging (IOI) in detecting the primary somatosensory cortex, providing a detailed assessment of their potential integration into surgical practice.ApproachData from nine patients undergoing tumor resection in the region of the somatosensory cortex were analyzed. Both IOI and ITI were employed simultaneously, with a specific focus on the areas identified as the primary somatosensory cortex (S1 region). The methodologies included a combination of imaging techniques during distinct phases of rest and stimulation, confirmed by electrophysiological monitoring of somatosensory evoked potentials to verify the functional areas identified by both imaging methods. The data were analyzed using a Fourier-based analytical framework to distinguish physiological signals from background noise.ResultsBoth ITI and IOI successfully generated reliable activity maps following median nerve stimulation. IOI showed greater consistency across various clinical scenarios, including those involving cortical tumors. Quantitative analysis revealed that IOI could more effectively differentiate genuine neuronal activity from artifacts compared with ITI, which was occasionally prone to false positives in the presence of cortical abnormalities.ConclusionsITI and IOI produce comparable functional maps with moderate agreement in Cohen’s kappa values. Their distinct physiological mechanisms suggest complementary use in specific clinical scenarios, such as cortical tumors or impaired neurovascular coupling. IOI excels in spatial resolution and mapping reliability, whereas ITI provides additional insights into metabolic changes and tissue properties, especially in pathological areas. Combined, these modalities could enhance the understanding and analysis of functional and pathological processes in complex neurosurgical cases.

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SPIE Journal Paper | 28 April 2023 Open Access

Fast and label-free intraoperative discrimination of malignant pancreatic tissue by attenuated total reflection infrared spectroscopy

Rimante Bandzeviciute, Gerald Steiner, Katja Liedel, Jonas Golde, Edmund Koch, Thilo Welsch, Christoph Kahlert, Daniel E. Stange, Marius Distler, Jürgen Weitz, Justinas Ceponkus, Valdas Sablinskas, Christian Teske

JBO, Vol. 28, Issue 04, 045004, (April 2023) https://doi.org/10.1117/12.10.1117/1.JBO.28.4.045004

KEYWORDS: Tissues, Tumors, Biological samples, Infrared spectroscopy, Resection, Spectroscopy, Attenuated total reflectance, Surgery, Crystals, Germanium

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Proceedings Article | 6 March 2023 Poster + Paper

Fiber-based ATR-IR spectroscopy for an intraoperative diagnosis of normal pancreatic tissue, pancreatitis, and malignant pancreatic lesions

Rimante Bandzeviciute, Gerald Steiner, Katja Liedel, Jonas Golde, Edmund Koch, Thilo Welsch, Christoph Kahlert, Daniel Stange, Marius Distler, Jürgen Weitz, Justinas Ceponkus, Valdas Sablinskas, Christian Teske

Proceedings Volume 12368, 123680H (2023) https://doi.org/10.1117/12.2647405

KEYWORDS: Tissues, Tumors, Infrared spectroscopy, Resection, Spectroscopy, Biological samples, Surgery, Attenuated total reflectance, Education and training

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SPIE Journal Paper | 25 July 2022 Open Access

Label-free differentiation of human pancreatic cancer, pancreatitis, and normal pancreatic tissue by molecular spectroscopy

Christian Teske, Christoph Kahlert, Thilo Welsch, Katja Liedel, Jürgen Weitz, Ortrud Uckermann, Gerald Steiner

JBO, Vol. 27, Issue 07, 075001, (July 2022) https://doi.org/10.1117/12.10.1117/1.JBO.27.7.075001

KEYWORDS: Tissues, Tumors, Infrared spectroscopy, Tissue optics, Pancreatic cancer, Principal component analysis, Imaging spectroscopy, Spectroscopy, Infrared imaging, Collagen

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Proceedings Article | 17 March 2020 Presentation

Intraoperative thermographic perfusion mapping in neurosurgery using regularized semiparametric regression (Conference Presentation)

Juliane Müller, Nico Hoffmann, Martin Oelschlägel, Christian Schnabel, Gerald Steiner, Edmund Koch, Stephan B. Sobottka, Gabriele Schackert, Matthias Kirsch

Proceedings Volume 11315, 113150N (2020) https://doi.org/10.1117/12.2549641

KEYWORDS: Signal detection, Brain mapping, Interference (communication), Signal processing, Thermal modeling, Blood circulation, Surgery, Tissues, Thermography, Cerebral blood flow

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Proceedings Article | 20 February 2018 Presentation + Paper

In situ detection of cancerous kidney tissue by means of fiber ATR-FTIR spectroscopy

Valdas Sablinskas, Martynas Velicka, Milda Pucetaite, Vidita Urboniene, Justinas Ceponkus, Rimante Bandzeviciute, Feliksas Jankevicius, Tatiana Sakharova, Olga Bibikova, Gerald Steiner

Proceedings Volume 10497, 1049713 (2018) https://doi.org/10.1117/12.2289393

KEYWORDS: Tissues, Spectroscopy, Kidney, Tissue optics, Cancer, Sensors, FT-IR spectroscopy, Infrared spectroscopy, Tumors

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The crucial goal of kidney-sparing surgical resection of a malignant tumor is complete removal of the cancerous tissue. The exact border between the cancerous and normal tissues is not always possible to identify by naked eye, therefore, a supplementary intraoperative diagnosis is needed. Unfortunately, intraoperative pathology methods used nowadays are time consuming and of inadequate quality rendering not definitive diagnosis. It has recently been shown that ATR-FTIR spectroscopy can be used for fast discrimination between cancerous and normal kidney tissues by analyzing the collected spectra of the tissue touch imprint smears. Most prominent differences are obtained in the wavenumber region from 950 cm^-1 to 1250 cm^-1, where the spectral bands due to the molecular vibrations of glycogen arise in the spectra of cancerous tissue smears. Such method of detection of cancerous tissue is limited by requirement to transfer the suspected tissue from the body to the FTIR instrument and stamp it on an ATR crystal of the spectrometer. We propose a spectroscopic tool which exploits the same principle of detection of cancerous cells as mentioned above, but does not require the tissue to be transferred from the body to the spectrometer. The portable spectrometer used in this design is equipped with fiber ATR probe and a sensitive liquid nitrogen cooled MCT detector. The design of the fiber probe allows the ATR tip to be changed easily in order to use only new sterilized tips for each measurement point of the tissue. It also enables sampling multiple areas of the suspected tissue with high lateral resolution which, in turn, increases accuracy with which the marginal regions between normal and cancerous tissues can be identified. Due to the loss of optical signal in the fiber probe the spectra have lower signal-to-noise ratio than in the case of standard ATR sampling setup. However, software for the spectral analysis used with the fiber probe design is still able to distinguish between cancerous and normal tissues with high accuracy.

Proceedings Article | 16 February 2017 Paper

Assignment of vibrational spectral bands of kidney tissue by means of low temperature SERS spectroscopy

M. Velicka, M. Radzvilaite, J. Ceponkus, V. Urboniene, M. Pucetaite, F. Jankevicius, G. Steiner, V. Sablinskas

Proceedings Volume 10068, 100681W (2017) https://doi.org/10.1117/12.2252907

KEYWORDS: Surface enhanced Raman spectroscopy, Tissues, Kidney, Raman spectroscopy, Cancer, Molecules, Nanoparticles, Silver, Spectroscopy

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SPIE Journal Paper | 4 May 2016 Open Access

Sandra Tamosaityte, Roberta Galli, Ortrud Uckermann, Kerim Sitoci-Ficici, Maria Koch, Robert Later, Gabriele Schackert, Edmund Koch, Gerald Steiner, Matthias Kirsch

JBO, Vol. 21, Issue 06, 061008, (May 2016) https://doi.org/10.1117/12.10.1117/1.JBO.21.6.061008

KEYWORDS: Raman spectroscopy, Injuries, Spinal cord, Tissues, Foam, Microscopy, Inflammation, Principal component analysis, Nervous system, Axons

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Proceedings Article | 7 March 2016 Presentation + Paper

Intra-operative on-line discrimination of kidney cancer from normal tissue by IR ATR spectroscopy of extracellular fluid

V. Urboniene, M. Velicka, J. Ceponkus, M. Pucetaite, F. Jankevicius, V. Sablinskas, G. Steiner

Proceedings Volume 9704, 97040E (2016) https://doi.org/10.1117/12.2213408

KEYWORDS: Tissues, Kidney, Cancer, Absorption, Infrared spectroscopy, Tissue optics, Surgery, Attenuated total reflectance, Principal component analysis, Spectroscopy

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Proceedings Article | 14 July 2015 Paper

Raman-based imaging uncovers the effects of alginate hydrogel implants in spinal cord injury

Roberta Galli, Sandra Tamosaityte, Maria Koch, Kerim Sitoci-Ficici, Robert Later, Ortrud Uckermann, Rudolf Beiermeister, Michael Gelinsky, Gabriele Schackert, Matthias Kirsch, Edmund Koch, Gerald Steiner

Proceedings Volume 9536, 95360Y (2015) https://doi.org/10.1117/12.2183165

KEYWORDS: Raman spectroscopy, Injuries, Tissues, Spinal cord, Collagen, Axons, Multiphoton microscopy, Nervous system, Statistical analysis, Therapeutics

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SPIE Journal Paper | 20 August 2014 Open Access

Identification of kidney tumor tissue by infrared spectroscopy of extracellular matrix

Vidita Urboniene, Milda Pucetaite, Feliksas Jankevicius, Arunas Zelvys, Valdas Sablinskas, Gerald Steiner

JBO, Vol. 19, Issue 08, 087005, (August 2014) https://doi.org/10.1117/12.10.1117/1.JBO.19.8.087005

KEYWORDS: Tumors, Tissues, FT-IR spectroscopy, Tissue optics, Kidney, Spectroscopy, Absorbance, Infrared spectroscopy, Imaging spectroscopy, Principal component analysis

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Proceedings Article | 12 March 2014 Paper

Intraoperative imaging of cortical perfusion by time-resolved thermography using cold bolus approach

Julia Hollmach, Christian Schnabel, Nico Hoffmann, Yordan Radev, Stephan Sobottka, Matthias Kirsch, Gabriele Schackert, Edmund Koch, Gerald Steiner

Proceedings Volume 9036, 903627 (2014) https://doi.org/10.1117/12.2043453

KEYWORDS: Thermography, Blood, Thermal modeling, Cameras, Imaging systems, Tissues, Temperature metrology, In vivo imaging, Heat treatments, Epilepsy

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Proceedings Article | 4 March 2014 Paper

Detection of cancerous kidney tissue areas by means of infrared spectroscopy of intercellular fluid

V. Urboniene, F. Jankevicius, A. Zelvys, G. Steiner, V. Sablinskas

Proceedings Volume 8939, 893913 (2014) https://doi.org/10.1117/12.2038539

KEYWORDS: Tumors, Tissues, Kidney, FT-IR spectroscopy, Infrared spectroscopy, Absorption, Tissue optics, Infrared radiation, Proteins, Cancer

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SPIE Journal Paper | 23 December 2013 Open Access

Effects of tissue fixation on coherent anti-Stokes Raman scattering images of brain

Roberta Galli, Ortrud Uckermann, Edmund Koch, Gabriele Schackert, Matthias Kirsch, Gerald Steiner

JBO, Vol. 19, Issue 07, 071402, (December 2013) https://doi.org/10.1117/12.10.1117/1.JBO.19.7.071402

KEYWORDS: Tissues, Brain, CARS tomography, Raman spectroscopy, Tumors, Neuroimaging, Tissue optics, Microscopy, Proteins, Imaging spectroscopy

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Proceedings Article | 17 June 2013 Paper

CARS and non-linear microscopy imaging of brain tumors

Roberta Galli, Ortrud Uckermann, Sandra Tamosaityte, Kathrin Geiger, Gabriele Schackert, Gerald Steiner, Edmund Koch, Matthias Kirsch

Proceedings Volume 8797, 87970E (2013) https://doi.org/10.1117/12.2031729

KEYWORDS: Tumors, Brain, Tissues, Microscopy, Nonlinear optics, Neuroimaging, In vivo imaging, Raman spectroscopy, Second-harmonic generation, CARS tomography

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Proceedings Article | 8 March 2013 Paper

Highly sensitive time-resolved thermography and multivariate image analysis of the cerebral cortex for intrasurgical diagnostics

Julia Hollmach, Nico Hoffmann, Christian Schnabel, Saskia Küchler, Stephan Sobottka, Matthias Kirsch, Gabriele Schackert, Edmund Koch, Gerald Steiner

Proceedings Volume 8565, 856550 (2013) https://doi.org/10.1117/12.2002342

KEYWORDS: Tumors, Thermography, Tissues, Wavelets, Discrete wavelet transforms, Image analysis, Cerebral cortex, Brain, Cameras, Diagnostics

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SPIE Journal Paper | 1 September 2011 Open Access

Infrared spectroscopic imaging of renal tumor tissue

Valdas Sablinskas, Vidita Urboniene, Justinas Ceponkus, Arvydas Laurinavicius, Darius Dasevicius, Feliksas Jankevicius, Vaiva Hendrixson, Edmund Koch, Gerald Steiner

JBO, Vol. 16, Issue 09, 096006, (September 2011) https://doi.org/10.1117/12.10.1117/1.3622292

KEYWORDS: Tumors, Tissues, Tissue optics, Spectroscopy, Imaging spectroscopy, Infrared spectroscopy, FT-IR spectroscopy, Infrared imaging, Infrared radiation, Absorbance

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Proceedings Article | 10 June 2011 Paper

Characterization of cytochrome c as marker for retinal cell degeneration by uv/vis spectroscopic imaging

Julia Hollmach, Julia Schweizer, Gerald Steiner, Lilla Knels, Richard H. Funk, Silko Thalheim, Edmund Koch

Proceedings Volume 8087, 80871F (2011) https://doi.org/10.1117/12.889466

KEYWORDS: Imaging spectroscopy, Spectroscopy, Absorption, Principal component analysis, Cell death, Data analysis, Visualization, Spectroscopes, Retina, Biomedical optics

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Proceedings Article | 23 February 2011 Paper

Infrared spectroscopic imaging of kidney tumor tissue

V. Sablinskas, G. Steiner, E. Koch, J. Ceponkus, M. Pucetaite, S. Strazdaite, V. Urboniene, F. Jankevicius

Proceedings Volume 7902, 790221 (2011) https://doi.org/10.1117/12.875258

KEYWORDS: Infrared spectroscopy, Infrared imaging, Spectroscopy, Imaging spectroscopy, Tissues, Tumors, Infrared radiation, Kidney, Tissue optics, Sensors

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SPIE Journal Paper | 1 January 2011 Open Access

Intraoperative imaging of cortical cerebral perfusion by time-resolved thermography and multivariate data analysis

Gerald Steiner, Stephan Sobottka, Edmund Koch, Gabriele Schackert, Matthias Kirsch

JBO, Vol. 16, Issue 1, 016001, (January 2011) https://doi.org/10.1117/12.10.1117/1.3528011

KEYWORDS: Principal component analysis, Thermography, Data analysis, Brain, Signal detection, Blood vessels, Temperature metrology, Blood, Neuroimaging, Blood circulation

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Proceedings Article | 12 February 2010 Paper

Bird sexing by Fourier transform infrared spectroscopy

Gerald Steiner, Thomas Bartels, Maria-Elisabeth Krautwald-Junghanns, Edmund Koch

Proceedings Volume 7560, 75600D (2010) https://doi.org/10.1117/12.841627

KEYWORDS: FT-IR spectroscopy, Absorption, Imaging spectroscopy, Spectroscopy, Proteins, Absorbance, Infrared imaging, Infrared radiation, Thermography, Eye

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Proceedings Article | 23 February 2009 Paper

Identification and classification of human neural stem cells by infrared spectroscopic imaging

G. Steiner, S. Küchler, E. Koch, R. Salzer, G. Schackert, M. Kirsch

Proceedings Volume 7169, 716911 (2009) https://doi.org/10.1117/12.808765

KEYWORDS: Infrared spectroscopy, Stem cells, Spectroscopy, Infrared radiation, Infrared imaging, Imaging spectroscopy, Neurons, Luminescence, Proteins, Algorithm development

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Proceedings Article | 21 February 2008 Paper

Infrared spectroscopic imaging with high spatial resolution and high sensitivity

Valdas Sablinskas, Gerald Steiner, Justinas Ceponkus, Reiner Salzer

Proceedings Volume 6869, 68690X (2008) https://doi.org/10.1117/12.768977

KEYWORDS: Free electron lasers, Spectroscopy, Infrared radiation, Molecules, Gold, Infrared spectroscopy, Infrared imaging, Spatial resolution, Mirrors, FT-IR spectroscopy

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Proceedings Article | 23 February 2006 Paper

Combination of SPR and PM-IRRAS for characterization and detection of biosensor arrays

Valdas Sablinskas, Gerald Steiner, Reiner Salzer

Proceedings Volume 6099, 60990I (2006) https://doi.org/10.1117/12.648360

KEYWORDS: Gold, Adsorption, Molecules, Aluminum, Spectroscopy, Image resolution, Self-assembled monolayers, Thin films, Infrared radiation, Spatial resolution

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Proceedings Article | 22 February 2006 Paper

Validation of IR-spectroscopic brain tumor classification

C. Beleites, G. Steiner, S. Sobottka, G. Schackert, R. Salzer

Proceedings Volume 6078, 60782V (2006) https://doi.org/10.1117/12.647496

KEYWORDS: Statistical modeling, Data modeling, Tissues, Brain, Tumors, Infrared spectroscopy, Infrared radiation, Spectroscopy, Statistical analysis, FT-IR spectroscopy

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Proceedings Article | 10 February 2006 Paper

Blood compatibility of artificial blood vessels probed by infrared spectroscopic imaging

Gerald Steiner, Manfred Maitz, Sibel Tunc, Reiner Salzer

Proceedings Volume 6093, 609302 (2006) https://doi.org/10.1117/12.644997

KEYWORDS: Proteins, Blood, FT-IR spectroscopy, Spectroscopy, Adsorption, Infrared spectroscopy, Imaging spectroscopy, Molecules, Atomic force microscopy, Silicon

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Proceedings Article | 9 May 2005 Paper

In vivo investigation of protein adsorption on implant surfaces

Reiner Salzer, Gerald Steiner, Sibel Tunc

Proceedings Volume 5768, (2005) https://doi.org/10.1117/12.598386

KEYWORDS: Proteins, Infrared spectroscopy, FT-IR spectroscopy, Raman spectroscopy, Spectroscopy, Adsorption, Imaging spectroscopy, Infrared imaging, In vivo imaging, Blood

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Proceedings Article | 21 July 2004 Paper

Health monitoring of biomaterials from molecular fingerprints

Reiner Salzer, Gerald Steiner, Christoph Krafft, Cordelia Zimmerer, Sibel Tunc

Proceedings Volume 5394, (2004) https://doi.org/10.1117/12.538374

KEYWORDS: Raman spectroscopy, Proteins, Spectroscopy, FT-IR spectroscopy, Imaging spectroscopy, Polymers, Sensors, Polymethylmethacrylate, Brain mapping, Imaging systems

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Proceedings Article | 1 August 2003 Paper

Optical biosensor array based on natural ion channels

Cordelia Zimmerer, Hans-Georg Braun, Marco Kitsche, Gerald Steiner, Steffen Friedrich, Reiner Salzer

Proceedings Volume 5047, (2003) https://doi.org/10.1117/12.508180

KEYWORDS: Ion channels, Polymers, Polymethylmethacrylate, Sensors, Imaging spectroscopy, FT-IR spectroscopy, Gold, Polymeric sensors, Receptors, Polymer thin films

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Proceedings Article | 1 August 2003 Paper

Spectral staining of tumor tissue by fiber optic FTIR spectroscopy

Reiner Salzer, Gerald Steiner, Angelique Kano, Tom Richter, Ralf Bergmann, Heike Rodig, Bernd Johannsen, Jens Kobelke

Proceedings Volume 5047, (2003) https://doi.org/10.1117/12.484153

KEYWORDS: Tumors, Silver, Tissues, Chalcogenides, FT-IR spectroscopy, Spectroscopy, Infrared radiation, Bromine, Optical fibers, Fiber optics

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Proceedings Article | 27 March 2002 Paper

Detection and grading of human gliomas by FTIR spectroscopy and a genetic classification algorithm

Gerald Steiner, R. Shaw, Lin-P'ing Choo-Smith, Wolfram Steller, Laryssa Shapoval, Gabriele Schackert, Stephan Sobottka, Reiner Salzer, Henry Mantsch

Proceedings Volume 4614, (2002) https://doi.org/10.1117/12.460789

KEYWORDS: Tissues, FT-IR spectroscopy, Spectroscopy, Brain, Diagnostics, Genetics, Genetic algorithms, Absorbance, Detection and tracking algorithms, Brain mapping

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Proceedings Article | 26 March 2002 Paper

Probing brain cancer by fiber optic FTIR spectroscopy

Gerald Steiner, Angelique Kano, Tom Richter, Ralf Bergmann, Heike Rodig, Jens Kobelke, Bernd Johannsen, Reiner Salzer

Proceedings Volume 4616, (2002) https://doi.org/10.1117/12.463822

KEYWORDS: Silver, Chalcogenides, Tumors, Tissues, FT-IR spectroscopy, Spectroscopy, Principal component analysis, Optical fibers, Infrared radiation, Fiber optics

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Proceedings Article | 8 May 2000 Paper

Surface-enhanced FTIR spectroscopy and surface plasmon resonance on biomembranes

Gerald Steiner, Reiner Salzer, Wolfgang Fischer, Christian Kuhne

Proceedings Volume 3918, (2000) https://doi.org/10.1117/12.384946

KEYWORDS: Germanium, Spectroscopy, Ion channels, Crystals, Proteins, Adsorption, FT-IR spectroscopy, Silver, Surface plasmons, Receptors

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Proceedings Article | 14 March 2000 Paper

Identification of cancer cells by a combination of FTIR spectroscopy and PET

Gerald Steiner, Tom Richter, Reiner Salzer, Ralf Bergmann, Heike Rodig, Bernd Johannsen

Proceedings Volume 3920, (2000) https://doi.org/10.1117/12.379587

KEYWORDS: Tissues, Positron emission tomography, Tumors, FT-IR spectroscopy, Cancer, Tissue optics, Spectroscopy, Principal component analysis, Connective tissue, Sensors

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Proceedings Article | 1 May 1998 Paper

Applications of surface-enhanced spectroscopic techniques to biosystems

Gerald Steiner, Christian Kuhne, Beate Leupolt, Wolfgang Fischer, Reiner Salzer

Proceedings Volume 3256, (1998) https://doi.org/10.1117/12.307072

KEYWORDS: Luminescence, Silver, Spectroscopy, Metals, Absorption, Crystals, Molecules, FT-IR spectroscopy, Molecular interactions, Surface enhanced Raman spectroscopy

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