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Ashly Jose

Post Doctoral Researcher

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SPIE Journal Paper | 8 March 2024 Open Access

Low-cost reversible tandem lens mesoscope for brain imaging in rodents

Ashly Jose, Pang Ying Cheung, Zahra Laouby, Frédérique Vanholsbeeck, Juliette E. Cheyne

NPh, Vol. 11, Issue 01, 014306, (March 2024) https://doi.org/10.1117/12.10.1117/1.NPh.11.1.014306

KEYWORDS: Brain, Neuroimaging, Spatial resolution, Imaging systems, Lenses, Cameras, In vivo imaging, Calcium, Image resolution, Brain imaging

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SignificanceThe development of imaging systems that are cost-efficient and modular is essential for modern neuroscience research.AimIn the current study, we designed, developed, and characterized a low-cost reversible tandem lens mesoscope for brain imaging in rodents.ApproachUsing readily available components, we assembled a robust imaging system that is highly efficient and cost-effective. We developed a mesoscope that offers high-resolution structural and functional imaging with cost-effective lenses and CMOS camera.ResultsThe reversible tandem lens configuration of the mesoscope offers two fields of view (FOVs), which can be achieved by swapping the objective and imaging lenses. The large FOV configuration of 12.6×10.5 mm provides a spatial resolution up to 4.92 μm, and the small FOV configuration of 6×5 mm provides a resolution of up to 2.46 μm. We demonstrate the efficiency of our system for imaging neuronal calcium activity in both rat and mouse brains in vivo.ConclusionsThe careful selection of the mesoscope components ensured its compactness, portability, and versatility, meaning that different types of samples and sample holders can be easily accommodated, enabling a range of different experiments both in vivo and in vitro. The custom-built reversible FOV mesoscope is cost-effective and was developed for under US$10,000 with excellent performance.

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