Heterogeneity plays an important role in medicine and biology, which can be investigated by exploiting single cell analysis (SCA). Among SCA methods, imaging cytometry allows the analysis of individual 2D and 3D spatial features. Here we present a femtosecond laser fabricated optofluidic automated platform encompassing a thermo-optic phase shifter, cylindrical lenses and a microfluidic network to generate and shift a dual-color patterned light sheet within a microchannel where the samples of interest flow. The device can be used as add-on and can provide an acquisition rate of about 1 cell/second, or subnuclear resolution at the single cell level.
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