Recent advances in optical imaging and spectroscopy of biological tissues facilitated groundbreaking discoveries in physiology of the lymphatic system of mammals. One important aspect is the dynamics of the lymphatic drainage between the eyes and the brain, which was potentially linked to a number of diseases. A mouse is a versatile model providing convenient in-vivo and ex-vivo studies of lymphatic drainage by multispectral optoacoustic tomography (MSOT) using near-infrared exogenous tracers. The accuracy of the in-vivo spectral umixing of chromohores by MSOT still requires further improvement to achieve required resolution. To achieve this goal, we studied factors such as the spectrum of wavelengths and skin pigmentation affecting the quantitative accuracy of MSOT tracking of the novel hybrid photoacoustic-fluorescent contrast agent QC-1/BSA/BODIPY injected into the lymph of C57 pigmented mice. We also compared performances of various spectral algorithms.
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